Department of Obstetrics and Gynecology, Weill Medical College of Cornell University, New York, NY, USA.
Obstet Gynecol. 2011 Oct;118(4):840-6. doi: 10.1097/AOG.0b013e31822da9e9.
To evaluate the influence of lactic acid on immune mediator release from vaginal epithelial cells.
The human vaginal epithelial cell line, VK2/E6E7, was cultured in the presence or absence of physiological concentrations of lactic acid, and in the presence or absence of the viral Toll-like receptor 3 agonist, poly (inosinic acid:cytidylic acid). Supernatants were assayed by enzyme-linked immunosorbent assay (ELISA) for interleukin (IL)-1β, IL-6, IL-8, IL-23, transforming growth factor (TGF)-β and secretory leukocyte protease inhibitor.
Vaginal epithelial cells spontaneously released IL-1β (25.9 pg/mL), IL-8 (1.0 ng/mL), TGF-β (175 pg/mL), and secretory leukocyte protease inhibitor (33.8 ng/mL). Only TGF-β production was marginally enhanced (49%) by addition of lactic acid alone. Poly (inosinic acid:cytidylic acid) by itself stimulated the release of IL-6 (305 pg/mL) and enhanced IL-8 production (2.8 ng/mL). The combination of poly (inosinic acid:cytidylic acid) and lactic acid markedly increased IL-8 production (5.0 ng/mL) and induced the release of IL-1β (96.2 pg/mL). The poly (inosinic acid:cytidylic acid)-mediated lactic acid effect on IL-1β and IL-8 release was abrogated when the lactic acid was neutralized or if acetic acid was substituted for lactic acid.
Lactic acid enhances the release of selective mediators from vaginal epithelial cells and stimulates antiviral immune responses.
评估乳酸对阴道上皮细胞免疫介质释放的影响。
在存在或不存在生理浓度乳酸的情况下，培养人阴道上皮细胞系 VK2/E6E7，并在存在或不存在病毒 Toll 样受体 3 激动剂聚（肌苷酸：胞苷酸）的情况下培养。通过酶联免疫吸附试验（ELISA）测定上清液中白细胞介素（IL）-1β、IL-6、IL-8、IL-23、转化生长因子（TGF）-β和分泌型白细胞蛋白酶抑制剂的含量。
阴道上皮细胞自发释放 IL-1β（25.9 pg/mL）、IL-8（1.0 ng/mL）、TGF-β（175 pg/mL）和分泌型白细胞蛋白酶抑制剂（33.8 ng/mL）。单独添加乳酸仅使 TGF-β 的产生略有增加（49%）。聚（肌苷酸：胞苷酸）本身刺激 IL-6 的释放（305 pg/mL）并增强 IL-8 的产生（2.8 ng/mL）。聚（肌苷酸：胞苷酸）和乳酸的组合显著增加了 IL-8 的产生（5.0 ng/mL）并诱导了 IL-1β 的释放（96.2 pg/mL）。中和乳酸或用乙酸代替乳酸可消除聚（肌苷酸：胞苷酸）介导的乳酸对 IL-1β 和 IL-8 释放的影响。
乳酸增强阴道上皮细胞选择性介质的释放，并刺激抗病毒免疫反应。
