Tianjin University of Science and Technology, Tianjin 300222, People's Republic of China.
Biotechnol Prog. 2012 Jan-Feb;28(1):215-22. doi: 10.1002/btpr.707. Epub 2011 Sep 20.
Water-soluble fullerene (C(60))-N-vinylpyrrolidone copolymers were prepared by the radical polymerization method. The structures of the copolymers were characterized by Fourier transform infrared, UV-Vis, (1)H NMR, (13)C NMR, gel permeation chromatography, thermogravimetric analyses, and scanning electron microscopy (SEM). The results presented show that C(60) and vinylpyrrolidone (VP) can be copolymerized under different conditions. With a constant benzoyl peroxide amount, C(60) contents in the copolymers increase with increasing initial C(60):VP reactant ratio. The assembly behavior of water-soluble C(60)-N-vinylpyrrolidone copolymers was investigated by SEM. The results show that the copolymers create morphology that is sphere-like. Fullerene-containing micro-/nano-sized copolymer fibers were prepared, for the first time, by electrospinning. The cytotoxicity to cancer cell lines of the copolymers was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide and confocal laser scanning microscope. The results show that copolymers exhibit better cytotoxicity against HeLa cells and mouse osteogenic sarcoma cells (cytotoxicity of copolymers is better than that of fullerene complex). The mechanism of fullerene-VP copolymerization was investigated for the first time.
采用自由基聚合法制备了水溶性富勒烯（C(60)）-N-乙烯基吡咯烷酮共聚物。通过傅里叶变换红外光谱、紫外-可见光谱、（1）H NMR、（13）C NMR、凝胶渗透色谱、热重分析和扫描电子显微镜（SEM）对共聚物的结构进行了表征。结果表明，C(60)和 N-乙烯基吡咯烷酮（VP）可以在不同条件下共聚。在过氧化二苯甲酰用量恒定的情况下，共聚物中 C(60)的含量随初始 C(60)：VP 反应物比例的增加而增加。通过 SEM 研究了水溶性 C(60)-N-乙烯基吡咯烷酮共聚物的组装行为。结果表明，共聚物形成了类似球体的形态。首次通过静电纺丝制备了含富勒烯的微/纳米尺寸共聚物纤维。通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐和共聚焦激光扫描显微镜评估了共聚物对癌细胞系的细胞毒性。结果表明，共聚物对 HeLa 细胞和小鼠成骨肉瘤细胞具有更好的细胞毒性（共聚物的细胞毒性优于富勒烯复合物）。首次研究了富勒烯-VP 共聚的机理。
