Environmental and Computational Chemistry Group, Faculty of Pharmaceutical Sciences, University of Cartagena, Cartagena, Colombia.
Nat Prod Res. 2012;26(12):1075-86. doi: 10.1080/14786419.2011.557376. Epub 2011 Sep 21.
Essential oils from Colombian plants were characterised by GC-MS, and assayed for anti-quorum sensing activity in bacteria sensor strains. Two major chemotypes were found for Lippia alba, the limonene-carvone and the citral (geranial-neral). For other species, the main components included α-pinene (Ocotea sp.), β-pinene (Swinglea glutinosa), cineol (Elettaria cardamomun), α-zingiberene (Zingiber officinale) and pulegone (Minthostachys mollis). Several essential oils presented promising inhibitory properties for the short chain AHL quorum sensing (QS) system, in Escherichia coli containing the biosensor plasmid pJBA132, in particular Lippia alba. Moderate activity as anti-QS using the same plasmid, were also found for selected constituents of essential oils studied here, such as citral, carvone and α-pinene, although solely at the highest tested concentration (250 µg mL(-1)). Only citral presented some activity for the long chain AHL QS system, in Pseudomonas putida containing the plasmid pRK-C12. In short, essential oils from Colombian flora have promising properties as QS modulators.
从哥伦比亚植物中提取的精油通过 GC-MS 进行了特征描述，并在细菌传感器菌株中检测了其抗群体感应活性。发现了两种主要的白里香化学型，即柠檬烯-蒈烯和柠檬醛（橙花醛-新橙花醛）。对于其他物种，主要成分包括α-蒎烯（Ocotea sp.）、β-蒎烯（Swinglea glutinosa）、桉油醇（Elettaria cardamomun）、α-姜烯（Zingiber officinale）和薄荷酮（Minthostachys mollis）。一些精油对含有生物传感器质粒 pJBA132 的大肠杆菌中的短链 AHL 群体感应（QS）系统表现出有希望的抑制特性，特别是白里香精油。用相同的质粒，我们还发现了这里研究的一些精油的选定成分，如柠檬醛、蒈烯和α-蒎烯，具有中等的抗 QS 活性，尽管仅在测试的最高浓度（250μg/mL）下。只有柠檬醛对含有质粒 pRK-C12 的假单胞菌中的长链 AHL QS 系统表现出一些活性。总之，哥伦比亚植物精油具有作为 QS 调节剂的有前途的特性。
