Bin Kong, Shi-Peng Zhao
Department of Coloproctology, Hebei Medical University, Shijiazhuang, China.
Hepatogastroenterology. 2011 Sep-Oct;58(110-111):1502-6. doi: 10.5754/hge11154. Epub 2011 Jul 15.
BACKGROUND/AIMS: To study the effects of water channel protein inhibitor acetazolamide on xenograft tumor growth of colon cancer in nude mice.
Setting up human colon cancer model in nude mice, mice were randomly divided into two groups as experimental group and control group. Acetazolamide was given at a volume of 0.1mL per mice (40mg/kg/d, ig) in experimental group, while the same volume of sterile saline was given in control group (ig). After 21 days, protein and m-RNA levels of AQP-1 in tumor tissues from two groups were detected respectively by Western blot and RT-PCR to evaluate the treatment effects. AQP-1, VEGF and CD34 expression was detected by immunohistochemistry, simultaneously.
Acetazolamide (40mg/kg/d, ig) significantly inhibited the xenograft tumor growth of colon cancer in nude mice. The inhibition rate was 88.28%. In comparison with the control group, AQP-1 protein and mRNA level were significantly reduced in the experimental group (p<0.01). AQP-1, VEGF and CD34 expression in experimental group were positively correlated between each other (p<0.01).
Acetazolamide can suppress the xenograft tumor growth by inhibiting the expression of AQP-1.
背景/目的：研究水通道蛋白抑制剂乙酰唑胺对裸鼠结肠癌移植瘤生长的影响。
建立裸鼠人结肠癌模型，将小鼠随机分为实验组和对照组。实验组给予乙酰唑胺，每只小鼠0.1mL（40mg/kg/d，灌胃），对照组给予等体积的无菌生理盐水（灌胃）。21天后，分别采用蛋白质印迹法和逆转录-聚合酶链反应检测两组肿瘤组织中AQP-1的蛋白质和mRNA水平，以评估治疗效果。同时采用免疫组织化学法检测AQP-1、血管内皮生长因子（VEGF）和CD34的表达。
乙酰唑胺（40mg/kg/d，灌胃）显著抑制裸鼠结肠癌移植瘤的生长，抑制率为88.28%。与对照组相比，实验组AQP-1的蛋白质和mRNA水平显著降低（p<0.01）。实验组中AQP-1、VEGF和CD34的表达两两之间呈正相关（p<0.01）。
乙酰唑胺可通过抑制AQP-1的表达来抑制移植瘤的生长。
