Karakuş Fuat, Eyol Ergül, Yılmaz Kadir, Ünüvar Songül
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, İnönü University, Malatya, Turkey.
Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ege University, İzmir, Turkey.
Afr Health Sci. 2018 Dec;18(4):1303-1310. doi: 10.4314/ahs.v18i4.51.
Metastasis is the leading cause of cancer deaths. Migration of tumor cells is an important stage in metastasis. Therefore, recent studies have focused on clarifying migration and migration-dependent cell functions such as angiogenesis, wound healing, and invasion.
In the present study, we aimed to investigate the effect of acetazolamide, which is a classical carbonic anhydrase inhibitor, on the cell viability, migration, and colony forming capacity of human LS174T colorectal cancer cells.
Three different cell culture techniques (MTT test, wound healing and clonogenic assay) were performed in this in vitro study on colorectal cancer cells.
Acetazolamide reduced the cell viability, migration and colony formation ability of cells depending on dose. There was no significant difference between the cells treated with acetazolamide with 1 µM dose and the control. However, it can be concluded that acetazolamide exerts its effect on human colorectal cancer cells at 10-1000 µM concentrations.
Acetazolamide was observed to significantly inhibit the cell viability, colony forming capacity, and migration ability in the culture medium of LS174T cells. This inhibitor effect of acetazolamide was observed to be dependent on the concentration in medium.
转移是癌症死亡的主要原因。肿瘤细胞的迁移是转移过程中的一个重要阶段。因此，最近的研究集中在阐明迁移以及与迁移相关的细胞功能，如血管生成、伤口愈合和侵袭。
在本研究中，我们旨在研究经典碳酸酐酶抑制剂乙酰唑胺对人LS174T结肠癌细胞的细胞活力、迁移和集落形成能力的影响。
在这项针对结肠癌细胞的体外研究中，采用了三种不同的细胞培养技术（MTT试验、伤口愈合试验和克隆形成试验）。
乙酰唑胺根据剂量降低了细胞的活力、迁移和集落形成能力。1 μM剂量的乙酰唑胺处理的细胞与对照组之间没有显著差异。然而，可以得出结论，乙酰唑胺在10 - 1000 μM浓度下对人结肠癌细胞发挥作用。
观察到乙酰唑胺显著抑制LS174T细胞培养基中的细胞活力、集落形成能力和迁移能力。观察到乙酰唑胺的这种抑制作用取决于培养基中的浓度。
