CAS Key Lab of Health Informatics, Shenzhen Key Laboratory of Cancer Nanotechnology, Institute of Biomedical and Health Engineering, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, People's Republic of China.
Nanotechnology. 2011 Oct 21;22(42):425502. doi: 10.1088/0957-4484/22/42/425502. Epub 2011 Sep 23.
A porous silicon microcavity (PSM) is highly sensitive to subtle interface changes due to its high surface area, capillary condensation ability and a narrow resonance peak (∼10 nm). Based on the well-defined optical properties of a PSM, we successfully fabricated a bacteria detection chip for molecular or subcellular analysis by surface modification using undecylenic acid (UA), and the specific recognition binding of vancomycin to the D-alanyl-D-alanine of bacteria. The red shift of the PSM resonance peak showed a good linear relationship with bacteria concentration ranging from 100 to 1000 bacteria ml( - 1) at the level of relative standard deviation of 0.994 and detection limit of 20 bacteria ml( - 1). The resulting PSM sensors demonstrated high sensitivity, good reproducibility, fast response and low cost for biosensing.
多孔硅微腔（PSM）由于其高表面积、毛细冷凝能力和窄共振峰（约 10nm），对细微的界面变化非常敏感。基于 PSM 的明确光学性质，我们通过使用十一烯酸（UA）进行表面修饰，成功地制造了用于分子或亚细胞分析的细菌检测芯片，以及万古霉素与细菌的 D-丙氨酰-D-丙氨酸的特异性识别结合。PSM 共振峰的红移与细菌浓度呈良好的线性关系，在相对标准偏差为 0.994 和检测限为 20 个细菌 ml(-1) 的水平下，细菌浓度范围为 100 至 1000 个细菌 ml(-1)。所得的 PSM 传感器表现出高灵敏度、良好的重现性、快速响应和低成本的生物传感。
