Zanner M A, Galey W R, Scaletti J V, Brahm J, Vander Jagt D L
Department of Biochemistry, University of New Mexico, School of Medicine, Albuquerque 87131.
Mol Biochem Parasitol. 1990 May;40(2):269-78. doi: 10.1016/0166-6851(90)90048-q.
The permeability properties of the human red cell membrane to various solutes are altered by malarial infection. In the present work we show that the permeability of the red cell membrane to water is also affected by the intraerythrocytic growth of the malaria parasite Plasmodium falciparum, whereas urea permeability appears unchanged. The data from infected cells show decreases in membrane surface area, cell volume, the osmotically active water fraction (Weff), and osmotic water permeability (Pf) as measured by stopped-flow spectroscopy. On the other hand, the data suggest an increase in diffusive water permeability (Pd) in infected cells with no change in urea permeability when measured by the continuous flow method. The decreased Pf/Pd ratio of infected cell membranes and its implications in the geometry of the red cell membrane water channel or pore are discussed.
疟原虫感染会改变人类红细胞膜对各种溶质的通透性。在本研究中，我们发现红细胞膜对水的通透性也会受到疟原虫恶性疟原虫在红细胞内生长的影响，而尿素通透性似乎未发生变化。通过停流光谱法测量，感染细胞的数据显示膜表面积、细胞体积、渗透活性水分数（Weff）和渗透水通透性（Pf）均降低。另一方面，通过连续流动法测量的数据表明，感染细胞的扩散水通透性（Pd）增加，而尿素通透性无变化。本文讨论了感染细胞膜Pf/Pd比值降低及其对红细胞膜水通道或孔隙几何结构的影响。
