利用光衍射层析成像技术对恶性疟原虫寄生的红细胞和原位血晶进行高分辨率三维成像。
High-resolution three-dimensional imaging of red blood cells parasitized by Plasmodium falciparum and in situ hemozoin crystals using optical diffraction tomography.
机构信息
Korea Advanced Institute of Science and Technology, Department of Physics, Daejeon 305-701, Republic of Korea.
出版信息
J Biomed Opt. 2014 Jan;19(1):011005. doi: 10.1117/1.JBO.19.1.011005.
Abstract
We present high-resolution optical tomographic images of human red blood cells (RBC) parasitized by malaria-inducing Plasmodium falciparum (Pf)-RBCs. Three-dimensional (3-D) refractive index (RI) tomograms are reconstructed by recourse to a diffraction algorithm from multiple two-dimensional holograms with various angles of illumination. These 3-D RI tomograms of Pf-RBCs show cellular and subcellular structures of host RBCs and invaded parasites in fine detail. Full asexual intraerythrocytic stages of parasite maturation (ring to trophozoite to schizont stages) are then systematically investigated using optical diffraction tomography algorithms. These analyses provide quantitative information on the structural and chemical characteristics of individual host Pf-RBCs, parasitophorous vacuole, and cytoplasm. The in situ structural evolution and chemical characteristics of subcellular hemozoin crystals are also elucidated.
摘要
我们呈现了人类受疟原虫感染的红细胞（RBC）的高分辨率光学层析图像。通过从多个具有不同照明角度的二维全息图中使用衍射算法，重建了三维折射率（RI）层析图像。Pf-RBC 的这些 3-D RI 层析图像详细显示了宿主 RBC 和入侵寄生虫的细胞和亚细胞结构。然后使用光学衍射层析成像算法系统地研究了寄生虫成熟的全无性红细胞内阶段（从环状体到滋养体到裂殖体阶段）。这些分析为单个宿主 Pf-RBC、寄生空泡和细胞质的结构和化学特性提供了定量信息。还阐明了亚细胞血红素晶体的原位结构演变和化学特性。
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