Jones A, Grace J, Hall B E
Department of Anatomical Pathology, Royal Prince Alfred Hospital, Sydney.
Pathology. 1990 Jan;22(1):5-9. doi: 10.3109/00313029009061417.
Twenty cases of non-Hodgkin's malignant lymphoma were examined using both flow cytometry (FCM) and static cytometry (SCM) DNA analysis to detect aneuploidic cell populations. FCM was performed on fresh cell suspensions whilst SCM was performed retrospectively on formalin-fixed, paraffin-embedded samples of the same tissue. A total of 34 aneuploidic cell lines were detected by FCM compared to a total of 41 detected by SCM. Of the 20 cases 14 showed DNA indexes within 10% of each other for each method, indicating a reasonable degree of comparability between the two methods. Of the 6 cases which did not show comparable DNA indexes, 2 displayed similar cell lines for both methods, but the major abnormal cell populations were of different ploidy by SCM as compared to FCM. A qualitative comparison is thus possible between these two cases. The results suggest that retrospectively applied SCM ploidy analysis of lymphoid tissue is comparable to FCM on fresh tissue samples and can thus be used in retrospective studies of both prognostic and diagnostic significance.
对20例非霍奇金恶性淋巴瘤病例进行了检测，采用流式细胞术（FCM）和静态细胞术（SCM）的DNA分析来检测非整倍体细胞群。FCM在新鲜细胞悬液上进行，而SCM则对同一组织的福尔马林固定、石蜡包埋样本进行回顾性分析。FCM共检测到34个非整倍体细胞系，而SCM共检测到41个。在这20例病例中，14例在每种方法下的DNA指数彼此相差在10%以内，表明两种方法具有合理程度的可比性。在6例未显示可比DNA指数的病例中，2例在两种方法下显示出相似的细胞系，但与FCM相比，SCM检测到的主要异常细胞群的倍性不同。因此，这两例病例之间可以进行定性比较。结果表明，对淋巴组织进行回顾性应用的SCM倍性分析与对新鲜组织样本进行的FCM相当，因此可用于具有预后和诊断意义的回顾性研究。
