García R, Bueno A, Feijoó E, Robledo M, Echezarreta G, Ortíz F, Benítez J, Sarasa J L, Rivas C
Department of Immunology, Fundacion Jiménez Díaz, Madrid, Spain.
Acta Oncol. 1994;33(6):621-5. doi: 10.3109/02841869409121772.
DNA content of 36-non-Hodgkin's lymphomas was analyzed by flow cytometry (FCM) and cytogenetics (CG), 21 in fresh and 15 in paraffin-embedded tissue. The results of both techniques were coincident in 60% of the fresh tissue samples and in 45% of the paraffin-embedded ones, the reason for this difference could be the poor resolution of DNA histograms from paraffin-embedded tissue. All samples judged as aneuploid by FCM were aneuploid also by CG. Some samples with a hyperdiploid population by CG gave a diploid population by FCM with a 'false' high DNA-synthesis (S) fraction. From a technical point of view, CG and FCM have to be performed on the same fresh tissue.
采用流式细胞术（FCM）和细胞遗传学（CG）分析了36例非霍奇金淋巴瘤的DNA含量，其中21例为新鲜组织，15例为石蜡包埋组织。两种技术的结果在60%的新鲜组织样本和45%的石蜡包埋样本中一致，这种差异的原因可能是石蜡包埋组织的DNA直方图分辨率较差。所有经FCM判定为非整倍体的样本经CG判定也为非整倍体。一些经CG检测为超二倍体群体的样本经FCM检测为二倍体群体，但DNA合成（S）分数“假”高。从技术角度来看，CG和FCM必须在同一新鲜组织上进行。
