Boskabady Mohammad Hossein, Tabanfar Hengameh
Department of Physiology and Pharmaceutical Research Centre, School of Medicine, Mashhad University of Medical Sciences, Mashhad, 9177948564, Iran Mehrdad Iranshahi.
Indian J Exp Biol. 2011 Sep;49(9):679-83.
The effects of three concentrations (2.5, 5 and 10 microg/ml) of aqueous-ethanolic extract of Z. multiflora bois, 10 nM chlorpheniramine, and saline on histamine (H1) receptors were tested on two groups of guinea pig tracheal chains [trachea incubated with indomethacin (Gr. 1), and indomethacin and propranolol (Gr. 2)]. The effective concentration of histamine causing 50% of maximum response (EC50) obtained in presence of chlorpheniramine in both groups, all concentrations of the extract in group 1 and its two higher concentrations in group 2 were significantly greater than those of saline. The values of concentration ratio minus one (CR-1) obtained in presence of all the three concentrations of the extract in group 1 and 10 microg/ml concentration in group 2 were significantly greater than those of chlorpheniramine. The values of EC50 obtained in presence of all the three concentrations of extract and CR-1 obtained in the presence of 2.5 and 5 microg/ml concentrations in group 2 were lower than group 1. There was not significant difference in maximum response obtained in presence of different concentrations of extract between two groups. There were parallel right ward shift in concentration response curves obtained in presence of all concentrations of the extract in both the groups. These results indicated an inhibitory effect of Z. multiflora at histamine H1 receptors.
在两组豚鼠气管链[用吲哚美辛孵育的气管（第1组），以及吲哚美辛和普萘洛尔（第2组）]上测试了三种浓度（2.5、5和10微克/毫升）的多花紫珠水乙醇提取物、10纳摩尔氯苯那敏和生理盐水对组胺（H1）受体的影响。在两组中，氯苯那敏存在时引起最大反应50%的组胺有效浓度（EC50），第1组中提取物的所有浓度以及第2组中其两个较高浓度均显著高于生理盐水。第1组中提取物的所有三种浓度以及第2组中10微克/毫升浓度存在时获得的浓度比减一（CR-1）值均显著高于氯苯那敏。第2组中提取物的所有三种浓度存在时获得的EC50值以及2.5和5微克/毫升浓度存在时获得的CR-1值均低于第1组。两组中不同浓度提取物存在时获得的最大反应无显著差异。两组中提取物所有浓度存在时获得的浓度反应曲线均有平行的右移。这些结果表明多花紫珠对组胺H1受体有抑制作用。
