Boskabady M H, Moghadas A
Department of Physiology, Ghlaem Medical Centre, Mashhad University of Medical Sciences, Mashhad 91735, Iran.
Phytomedicine. 2004 Jul;11(5):411-5. doi: 10.1016/j.phymed.2003.06.001.
The antihistaminic effects of aqueous and macerated extracts, essential oil, 20 nM chlorpheniramine, and saline were tested by performing the cumulative log concentration-response curves of histamine-induced contraction of isolated guinea pig tracheal chains incubated with three different conditions including: (1) 1.4 microM indomethacin, (2) indomethacin, 1 microM propranolol, and 10 nM atropine, and (3) indomethacin and propranolol (for each group n = 8). The results showed clear parallel rightward shifts in histamine-response curves obtained in the presence of macerated extract in group 2, aqueous extract in group 3, and essential oil in groups 2 and 3 experiments compared with the curves obtained in the presence of saline. The EC50 (effective concentration of histamine causing 50% of maximum response) obtained in the presence of essential oil, extracts, and chlorpheniramine in all three sets of experiments were significantly higher than that of saline (P<0.05 to p<0.001). The maximum response obtained in the presence of aqueous extract in group 3 compared to group I and that of macerated extract in group 2 compared to the other two sets of experiments were improved. These results indicated a competitive antagonistic effect of Bunium persicum at histamine H1 receptors.
通过对组胺诱导的豚鼠离体气管链收缩进行累积对数浓度-反应曲线测试，来检验水提取物、浸渍提取物、精油、20 nM氯苯那敏和生理盐水的抗组胺作用，实验在三种不同条件下进行，包括：(1) 1.4 μM吲哚美辛；(2) 吲哚美辛、1 μM普萘洛尔和10 nM阿托品；(3) 吲哚美辛和普萘洛尔（每组n = 8）。结果显示，与生理盐水组相比，在第2组浸渍提取物、第3组水提取物以及第2组和第3组精油存在的情况下所获得的组胺反应曲线均明显平行右移。在所有三组实验中，精油、提取物和氯苯那敏存在时所获得的组胺EC50（引起最大反应50%的组胺有效浓度）均显著高于生理盐水组（P<0.05至P<0.001）。与第1组相比，第3组水提取物存在时所获得的最大反应有所改善，与其他两组实验相比，第2组浸渍提取物存在时所获得的最大反应也有所改善。这些结果表明，波斯孜然在组胺H1受体上具有竞争性拮抗作用。
