大马士革蔷薇对豚鼠气管链的松弛作用及其可能机制

Relaxant effects of Rosa damascena on guinea pig tracheal chains and its possible mechanism(s).

作者信息

Boskabady M H, Kiani S, Rakhshandah H

机构信息

Department of Physiology, Ghaem Medical Centre, Mashhad University of Medical Sciences, Mashhad 91735, Iran.

出版信息

J Ethnopharmacol. 2006 Jul 19;106(3):377-82. doi: 10.1016/j.jep.2006.01.013. Epub 2006 Feb 28.

DOI:10.1016/j.jep.2006.01.013

PMID:16504433

Abstract

Several therapeutic effects including hypnotic, antispasmodic, treatment of abdominal and chest pain and strengthening the heart have been described for the flowers of Rosa damascena. Therefore in the present study, the relaxant effects of ethanolic extract and essential oils of Rosa damascena on tracheal chains of guinea pigs were examined. The relaxant effects of four cumulative concentrations of ethanolic extract (0.25, 0.5, 0.75, and 1.0g%) and essential oils (0.25, 0.5, 0.75, and 1.0vol.%) in comparison with saline as negative control and four cumulative concentrations of theophylline (0.25, 0.5, 0.75, and 1.0mM) were examined by their relaxant effects on precontracted tracheal chains of guinea pig by 60mM KCl (group 1, n=5) and 10microM methacholine in two different conditions including: non-incubated tissues (group 2, n=8) and incubated tissues with 1microM propranolol and 1microM chlorpheniramine (group 3, n=5). In group 1 experiments two final concentrations of essential oil and theophylline and only final concentration of ethanolic extract showed relaxant effects compared to that of saline (p<0.01-0.001). In group 2 three higher concentrations of ethanolic extract and theophylline and all concentrations of essential oil showed concentration dependent relaxant effects compared to that of saline (p<0.05-0.001). In addition, the effect of 0.25 and 0.5g% of essential oils in group 2 was significantly higher than those of theophylline and ethanolic extract (p<0.01 for all cases). However, in group 3 experiments the extract and essential oil of Rosa damascena did not show any significant relaxant effect. There were significant correlations between the relaxant effects and concentrations for ethanolic extract and essential oil and theophylline in groups 1 and 2. These results showed a potent relaxant effect of Rosa damascena on tracheal chains of guinea pigs that was comparable to that of theophylline at concentrations used.

摘要

大马士革蔷薇花具有多种治疗作用，包括催眠、解痉、治疗腹部和胸痛以及增强心脏功能。因此，在本研究中，检测了大马士革蔷薇乙醇提取物和精油对豚鼠气管链的舒张作用。将乙醇提取物的四个累积浓度（0.25、0.5、0.75和1.0g%）和精油的四个累积浓度（0.25、0.5、0.75和1.0vol.%）与作为阴性对照的生理盐水以及茶碱的四个累积浓度（0.25、0.5、0.75和1.0mM）进行比较，通过它们对60mM氯化钾预收缩的豚鼠气管链（第1组，n = 5）和10μM乙酰甲胆碱在两种不同条件下的舒张作用进行检测，这两种条件包括：未孵育的组织（第2组，n = 8）和用1μM普萘洛尔和1μM氯苯那敏孵育的组织（第3组，n = 5）。在第1组实验中，与生理盐水相比，精油和茶碱的两个终浓度以及乙醇提取物的仅终浓度显示出舒张作用（p < 0.01 - 0.001）。在第2组中，与生理盐水相比，乙醇提取物和茶碱的三个较高浓度以及精油的所有浓度均显示出浓度依赖性舒张作用（p < 0.05 - 0.001）。此外，第2组中0.25和0.5g%精油的作用显著高于茶碱和乙醇提取物（所有情况p < 0.01）。然而，在第3组实验中，大马士革蔷薇提取物和精油未显示出任何显著的舒张作用。第1组和第2组中乙醇提取物、精油和茶碱的舒张作用与浓度之间存在显著相关性。这些结果表明，大马士革蔷薇对豚鼠气管链具有强大的舒张作用，在所用浓度下与茶碱相当。