一种含有M13复制起点的酵母-大肠杆菌穿梭载体。
A yeast-Escherichia coli shuttle vector containing the M13 origin of replication.
作者信息
Rhodes N, Company M, Errede B
机构信息
Department of Chemistry, University of North Carolina, Chapel Hill 27599-3290.
出版信息
Plasmid. 1990 Mar;23(2):159-62. doi: 10.1016/0147-619x(90)90036-c.
PMID:2194231
Abstract
A yeast-Escherichia coli shuttle vector containing the M13 origin of replication has been constructed. This vector allows selection and replication in both Saccharomyces cerevisiae and E. coli, as well as single-stranded packaging from E. coli upon infection with a helper phage. The presence of a polylinker with various unique restriction sites facilitates the cloning of desired genes.
摘要
构建了一种含有M13复制起点的酵母-大肠杆菌穿梭载体。该载体允许在酿酒酵母和大肠杆菌中进行选择和复制，并且在被辅助噬菌体感染后能在大肠杆菌中进行单链包装。带有各种独特限制酶切位点的多克隆位点的存在便于克隆所需基因。
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