杰基尔，一种噬菌体 - 质粒穿梭载体家族。
Jekyll, a family of phage-plasmid shuttle vectors.
作者信息
Burmeister M, Lehrach H
机构信息
European Molecular Biology Laboratory, Heidelberg, F.R.G.
出版信息
Gene. 1988 Dec 15;73(1):245-50. doi: 10.1016/0378-1119(88)90331-9.
A series of shuttle vectors has been constructed, which consist of a plasmid carrying a polylinker sequence and an M13 origin integrated into a lambda vector. A short direct repeat flanking the plasmid allows plasmid excision by homologous recombination. Sequences are cloned into unique restriction sites within the plasmid, and can be recovered either in phage or plasmid form, or can be packaged further as single-stranded DNA phage. These vectors therefore combine the efficiency of phage lambda cloning and screening with the ease of handling or analysing plasmid or M13 clones.
已经构建了一系列穿梭载体，它们由携带多克隆位点序列和整合到λ载体中的M13起源的质粒组成。质粒侧翼的短直接重复序列允许通过同源重组切除质粒。序列被克隆到质粒内的独特限制酶切位点，可以以噬菌体或质粒形式回收，或者可以进一步包装成单链DNA噬菌体。因此，这些载体将λ噬菌体克隆和筛选的效率与处理或分析质粒或M13克隆的简便性结合在一起。
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