阿尔茨海默病患者血液样本中 CCL5[RANTES]mRNA 表达降低。

Decreased mRNA expression of CCL5 [RANTES] in Alzheimer's disease blood samples.

机构信息

Alzheimer Center and Department of Neurology, VU University Medical Center, Amsterdam, The Netherlands.

出版信息

Clin Chem Lab Med. 2011 Sep 23;50(1):61-5. doi: 10.1515/CCLM.2011.731.

DOI:10.1515/CCLM.2011.731

PMID:21942811

Abstract

BACKGROUND

A recent study reported that an 18-analyte multiplexed plasma panel of signaling proteins differentiated Alzheimer's disease (AD) from controls. This study measured mRNA expression for nine of these promising bio-markers in 23 AD patients and 23 age- and sex-matched controls.

METHODS

Total RNA was isolated from PaxGene RNA tubes. Relative mRNA expression levels of CCL5 [RANTES], CSF1, ICAM1, IGFBP6, IL1A, IL3, IL8, PDGFB and TNF were determined by Q-RT-PCR, with GAPDH as housekeeping gene.

RESULTS

A panel of five markers (CCL5, CSF1, ICAM1, IL8, TNF) with detectable expression levels in all individuals differed between AD patients and controls (p interaction <0.10). Especially, the relative expression level of CCL5 was lower in AD patients than in controls (p<0.005). Across groups, levels of both CCL5 and TNF were correlated to CSF levels of τ (r=0.39, r=0.32), pτ-181 (r=0.38, r=0.33), and MMSE (r=-0.31, r=-0.33, all p<0.05).

CONCLUSIONS

The measured panel, and especially CCL5, could aid in the differentiation of AD from controls.

摘要

背景

最近的一项研究报告称，一种由 18 种分析物组成的信号蛋白多重血浆面板可以区分阿尔茨海默病（AD）与对照。本研究在 23 名 AD 患者和 23 名年龄和性别匹配的对照中测量了这 9 个有前途的生物标志物的 mRNA 表达。

方法

从 PaxGene RNA 管中分离总 RNA。通过 Q-RT-PCR 测定 CCL5[RANTES]、CSF1、ICAM1、IGFBP6、IL1A、IL3、IL8、PDGFB 和 TNF 的相对 mRNA 表达水平，GAPDH 作为管家基因。

结果

一个在所有个体中都具有可检测表达水平的五个标志物（CCL5、CSF1、ICAM1、IL8、TNF）在 AD 患者和对照组之间存在差异（p 交互<0.10）。特别是，AD 患者的 CCL5 相对表达水平低于对照组（p<0.005）。在各组中，CCL5 和 TNF 的水平均与 CSF 中 τ（r=0.39，r=0.32）、pτ-181（r=0.38，r=0.33）和 MMSE（r=-0.31，r=-0.33，均 p<0.05）呈正相关。