Laboratorio di Tossicologia, Nuovo Ospedale Civile S. Agostino Estense, Modena, Italy.
Clin Chem Lab Med. 2011 Sep 26;50(1):139-46. doi: 10.1515/CCLM.2011.718.
Aution Max AX-4030, a test strip analyzer recently introduced to the market, represents an upgrade of the Aution Max AX-4280 widely employed for urinalysis. This new instrument model can allocate two different test strips at the same time. In the present study the two instruments have been compared together with the usage of Uriflet 9UB and the recently produced Aution Sticks 10PA urine strips, the latter presenting an additional test area for the measurement of urinary creatinine.
Imprecision and correlation between instruments and strips have been evaluated for chemical-physical parameters. Accuracy was evaluated for protein, glucose and creatinine by comparing the semi-quantitative results to those obtained by quantitative methods. The well-known interference effect of high ascorbic acid levels on urine glucose test strip determination was evaluated, ascorbic acid influence was also evaluated on protein and creatinine determination.
The two instruments have demonstrated comparable performances: precision and correlation between instruments and strips, evaluated for chemical-physical parameters, were always good. Furthermore, accuracy was always very good: results of protein and glucose semi-quantitative measurements resulted to be highly correlated with those obtained by quantitative methods. Moreover, the semi-quantitative measurements of creatinine, employing Aution Sticks 10PA urine strips, were highly comparable with quantitative results.
10PA urine strips are eligible for urine creatinine determination with the possibility of correcting urinalysis results for urinary creatinine concentration, whenever necessary and calculating the protein creatinine ratio. Further studies should be carried out to evaluate effectiveness and appropriateness of the usage of creatinine semi-quantitative analysis.
Aution Max AX-4030 是一款最近推向市场的测试条分析仪，它是广泛应用于尿液分析的 Aution Max AX-4280 的升级版。这款新仪器模型可以同时分配两种不同的测试条。在本研究中，将这两种仪器与 Uriflet 9UB 和最近生产的 Aution Sticks 10PA 尿液条一起使用进行了比较，后者在用于测量尿肌酐的附加测试区域提供了额外的测试条。
评估了仪器和测试条的化学物理参数的不精密度和相关性。通过将半定量结果与定量方法获得的结果进行比较，评估了蛋白质、葡萄糖和肌酐的准确性。评估了高浓度抗坏血酸对尿液葡萄糖测试条测定的已知干扰影响，还评估了抗坏血酸对蛋白质和肌酐测定的影响。
这两种仪器表现出可比较的性能：化学物理参数的仪器和测试条之间的精密度和相关性始终良好。此外，准确性始终非常好：蛋白质和葡萄糖半定量测量结果与定量方法获得的结果高度相关。此外，使用 Aution Sticks 10PA 尿液条进行的肌酐半定量测量与定量结果高度可比。
10PA 尿液条有资格用于尿液肌酐的测定，具有根据需要校正尿液肌酐浓度的尿液分析结果的能力，并计算蛋白质肌酐比。应进一步开展研究，以评估肌酐半定量分析的有效性和适当性。
