Wang Li-Shan, Chung Joo Eun, Kurisawa Motoichi
a Institute of Bioengineering and Nanotechnology , 31 Biopolis Way , The Nanos , 138669 , Singapore.
J Biomater Sci Polym Ed. 2012;23(14):1793-806. doi: 10.1163/156856211X598193. Epub 2012 May 8.
We report an injectable hydrogel system with tunable stiffness for controlling the proliferation rate of human fibroblasts (HFF-1) in both two-dimensional (2D) and three-dimensional (3D) culture environments for potential use as a wound dressing material. The hydrogel composed of gelatin-hydroxyphenylpropionic acid (Gtn-HPA) conjugate was formed by the oxidative coupling of HPA moieties catalyzed by hydrogen peroxide (H2O2) and horseradish peroxidase (HRP). The stiffness of the hydrogels was controlled well by varying the H2O2 concentration. The effects of hydrogel stiffness on the proliferation rate of HFF-1 in both 2D and 3D were investigated. We found that the proliferation rate of HFF-1 using Gtn-HPA hydrogels was strongly dependent on the hydrogel stiffness, with a dimensionality-specific response. In the 2D studies, the HFF-1 exhibited a higher proliferation rate when the stiffness of the hydrogel was increased. In contrast, the HFF-1 cultured inside the hydrogel remained non-proliferative for 12 days before a stiffness-dependent proliferation profile was shown. The proliferation rate decreased with an increase in stiffness of the hydrogel in a 3D culture environment, unlike in a 2D environment.
我们报道了一种具有可调刚度的可注射水凝胶系统，用于在二维（2D）和三维（3D）培养环境中控制人成纤维细胞（HFF-1）的增殖速率，有望用作伤口敷料材料。由明胶-羟基苯丙酸（Gtn-HPA）共轭物组成的水凝胶是通过过氧化氢（H2O2）和辣根过氧化物酶（HRP）催化的HPA部分的氧化偶联形成的。通过改变H2O2浓度可以很好地控制水凝胶的刚度。研究了水凝胶刚度对2D和3D环境中HFF-1增殖速率的影响。我们发现，使用Gtn-HPA水凝胶时，HFF-1的增殖速率强烈依赖于水凝胶刚度，并具有维度特异性响应。在2D研究中，当水凝胶刚度增加时，HFF-1表现出更高的增殖速率。相比之下，在显示出刚度依赖性增殖曲线之前，在水凝胶内部培养的HFF-1在12天内保持不增殖状态。与2D环境不同，在3D培养环境中，增殖速率随着水凝胶刚度的增加而降低。
