Smallbone Kieran, Malys Naglis, Messiha Hanan L, Wishart Jill A, Simeonidis Evangelos
Manchester Centre for Integrative Systems Biology, The University of Manchester, Manchester, United Kingdom.
Methods Enzymol. 2011;500:355-70. doi: 10.1016/B978-0-12-385118-5.00018-9.
In this chapter, we describe the steps needed to create a kinetic model of a metabolic pathway based on kinetic data from experimental measurements and literature review. Our methodology is presented by utilizing the example of trehalose metabolism in yeast. The biology of the trehalose cycle is briefly reviewed and discussed.
在本章中,我们描述了根据实验测量和文献综述中的动力学数据创建代谢途径动力学模型所需的步骤。我们以酵母中的海藻糖代谢为例介绍了我们的方法。简要回顾并讨论了海藻糖循环的生物学特性。