Eagle Susan, Brophy Colleen M, Komalavilas Padmini, Hocking Kyle, Putumbaka Gowthami, Osgood Michael, Sexton Kevin, Leacche Marzia, Cheung-Flynn Joyce
Department of Anesthesiology, Vanderbilt University Medical Center, Nashville, Tennessee, USA.
Am Surg. 2011 Jul;77(7):922-8.
Marking human saphenous vein graft (HSV) with a surgical skin marker to prevent twisting on implantation is a common practice in peripheral and coronary artery bypass procedures. This study is designed to examine the effects of surgical skin markers on the HSV smooth muscle and endothelial functional responses. De-identified HSV remnants were collected during peripheral and coronary artery bypass procedures. Physiologic responses of the HSV were measured using a muscle bath. Veins that were marked with surgical skin markers intraoperatively generated significantly less contractile force to depolarizing KCl (110 mM) and receptor-mediated contractile agonists than unmarked HSV, suggesting that surgical skin markers impaired HSV smooth muscle contractility. To directly access the effects of chemical components in the surgical skin markers, unmarked HSV was exposed to isopropyl alcohol (a solvent commonly used in surgical skin markers) or methylene blue (a dye). Smooth muscle contractility was significantly reduced by isopropyl alcohol and methylene blue. Endothelial-dependent relaxation to carbachol was significantly reduced after exposure to surgical skin markers. Our data demonstrated that marking HSV with surgical skin markers reduces smooth muscle and endothelial functional viability.
在周围血管和冠状动脉搭桥手术中，使用手术皮肤标记物标记人隐静脉移植物（HSV）以防止植入时扭转是一种常见做法。本研究旨在检验手术皮肤标记物对HSV平滑肌和内皮功能反应的影响。在周围血管和冠状动脉搭桥手术期间收集了身份不明的HSV残余物。使用肌肉浴测量HSV的生理反应。术中用手术皮肤标记物标记的静脉对去极化氯化钾（110 mM）和受体介导的收缩激动剂产生的收缩力明显低于未标记的HSV，这表明手术皮肤标记物损害了HSV平滑肌的收缩性。为了直接了解手术皮肤标记物中化学成分的影响，将未标记的HSV暴露于异丙醇（手术皮肤标记物中常用的溶剂）或亚甲蓝（一种染料）中。异丙醇和亚甲蓝显著降低了平滑肌收缩性。暴露于手术皮肤标记物后，对卡巴胆碱的内皮依赖性舒张作用显著降低。我们的数据表明，用手术皮肤标记物标记HSV会降低平滑肌和内皮的功能活力。
