Benedetti G, De Santis P, Morosetti S
Department of Chemistry, University of Rome I, Italy.
J Biomol Struct Dyn. 1990 Jun;7(6):1269-77. doi: 10.1080/07391102.1990.10508564.
The secondary structures of Tetrahymena thermophila rRNA IVS sequence involved in the self-splicing reactions, are theoretically investigated with a refined computer method previously proposed, able to select a set of the deepest free energy RNA secondary structures under constraints of model hypotheses and experimental evidences. The secondary structures obtained are characterized by the close proximity of self-reactions sites and account for double mutations experiments, and differential digestion data.
利用先前提出的一种改进的计算机方法，从理论上研究了嗜热四膜虫rRNA IVS序列中参与自我剪接反应的二级结构。该方法能够在模型假设和实验证据的约束下，选择一组具有最低自由能的RNA二级结构。所获得的二级结构的特点是自我反应位点紧密相邻，并能解释双突变实验和差异消化数据。
