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通过分子的反应性简化形式所示的嗜热四膜虫核酶催化核心的结构。

Structure of the catalytic core of the Tetrahymena ribozyme as indicated by reactive abbreviated forms of the molecule.

作者信息

Joyce G F, Inoue T

机构信息

Salk Institute for Biological Studies, San Diego, CA 92138.

出版信息

Nucleic Acids Res. 1987 Dec 10;15(23):9825-40. doi: 10.1093/nar/15.23.9825.

DOI:10.1093/nar/15.23.9825
PMID:3697083
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC306534/
Abstract

The precursor rRNA of Tetrahymena thermophila contains a group I intervening sequence (IVS) that catalyzes its own excision to yield mature rRNA. The excised IVS catalyzes a number of cleavage/ligation reactions that are analogous to the transesterification reactions of splicing. We examined the behavior of a variety of 3'-truncated forms of the IVS and found several abbreviated molecules that retained catalytic activity. The reactivity of these molecules indicates that the site at which cleavage/ligation occurs lies in close proximity to all of the conserved sequence elements within the catalytic core of the IVS.

摘要

嗜热四膜虫的前体核糖体RNA（rRNA）含有一个I型内含子序列（IVS），该序列催化自身切除以产生成熟的rRNA。切除的IVS催化许多切割/连接反应，这些反应类似于剪接的转酯反应。我们研究了IVS的多种3'端截短形式的行为，发现了几种保留催化活性的缩短分子。这些分子的反应活性表明，切割/连接发生的位点紧邻IVS催化核心内的所有保守序列元件。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1534/306534/308ae55a4fd5/nar00267-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1534/306534/8a4d75f007cd/nar00267-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1534/306534/8876c9f6d5ef/nar00267-0215-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1534/306534/e060ea0922dd/nar00267-0216-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1534/306534/9efe9a03e052/nar00267-0217-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1534/306534/308ae55a4fd5/nar00267-0218-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1534/306534/8a4d75f007cd/nar00267-0211-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1534/306534/8876c9f6d5ef/nar00267-0215-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1534/306534/e060ea0922dd/nar00267-0216-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1534/306534/9efe9a03e052/nar00267-0217-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1534/306534/308ae55a4fd5/nar00267-0218-a.jpg

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1
Structure of the catalytic core of the Tetrahymena ribozyme as indicated by reactive abbreviated forms of the molecule.通过分子的反应性简化形式所示的嗜热四膜虫核酶催化核心的结构。
Nucleic Acids Res. 1987 Dec 10;15(23):9825-40. doi: 10.1093/nar/15.23.9825.
2
Autocatalytic cyclization of an excised intervening sequence RNA is a cleavage-ligation reaction.切除的居间序列RNA的自催化环化是一种切割-连接反应。
Nature. 1983;301(5901):578-83. doi: 10.1038/301578a0.
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Secondary structure of the Tetrahymena ribosomal RNA intervening sequence: structural homology with fungal mitochondrial intervening sequences.嗜热四膜虫核糖体RNA间隔序列的二级结构：与真菌线粒体间隔序列的结构同源性。
Proc Natl Acad Sci U S A. 1983 Jul;80(13):3903-7. doi: 10.1073/pnas.80.13.3903.
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Catalysis of splicing-related reactions between dinucleotides by a ribozyme.核酶催化二核苷酸之间的剪接相关反应。
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Sequence requirements for self-splicing of the Tetrahymena thermophila pre-ribosomal RNA.嗜热栖热菌前核糖体RNA自我剪接的序列要求。
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In vitro splicing of the ribosomal RNA precursor of Tetrahymena: involvement of a guanosine nucleotide in the excision of the intervening sequence.嗜热四膜虫核糖体RNA前体的体外剪接：鸟苷酸在间隔序列切除中的作用。
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Ribozyme inhibitors: deoxyguanosine and dideoxyguanosine are competitive inhibitors of self-splicing of the Tetrahymena ribosomal ribonucleic acid precursor.核酶抑制剂：脱氧鸟苷和双脱氧鸟苷是嗜热四膜虫核糖体核糖核酸前体自我剪接的竞争性抑制剂。
Biochemistry. 1986 Aug 12;25(16):4473-7. doi: 10.1021/bi00364a001.

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本文引用的文献

1
Conservation of RNA secondary structures in two intron families including mitochondrial-, chloroplast- and nuclear-encoded members.两个内含子家族中RNA二级结构的保守性，包括线粒体、叶绿体和核编码成员。
EMBO J. 1983;2(1):33-8. doi: 10.1002/j.1460-2075.1983.tb01376.x.
2
The intervening sequence excised from the ribosomal RNA precursor of Tetrahymena contains a 5-terminal guanosine residue not encoded by the DNA.从四膜虫核糖体RNA前体切除的居间序列含有一个DNA未编码的5'-末端鸟苷残基。
Nucleic Acids Res. 1982 May 11;10(9):2823-38. doi: 10.1093/nar/10.9.2823.
3
Bacteriophage SP6-specific RNA polymerase. I. Isolation and characterization of the enzyme.
尽管P5元件的大片段延伸被去除，但嗜热四膜虫I组内含子仍保留催化活性。
Nucleic Acids Res. 1989 Oct 11;17(19):7879-89. doi: 10.1093/nar/17.19.7879.
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Cloning, in vitro transcription, and biological activity of Escherichia coli 23S ribosomal RNA.大肠杆菌23S核糖体RNA的克隆、体外转录及生物活性
Nucleic Acids Res. 1990 Jun 25;18(12):3515-20. doi: 10.1093/nar/18.12.3515.
5
The mechanism of group I self-splicing: an internal guide sequence can be provided in trans.I类自我剪接的机制：内部引导序列可通过反式提供。
EMBO J. 1990 Jun;9(6):1923-8. doi: 10.1002/j.1460-2075.1990.tb08319.x.
噬菌体SP6特异性RNA聚合酶。I. 酶的分离与特性鉴定。
J Biol Chem. 1982 May 25;257(10):5772-8.
4
Comparison of fungal mitochondrial introns reveals extensive homologies in RNA secondary structure.真菌线粒体内含子的比较揭示了RNA二级结构中广泛的同源性。
Biochimie. 1982 Oct;64(10):867-81. doi: 10.1016/s0300-9084(82)80349-0.
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Making ends meet: a model for RNA splicing in fungal mitochondria.收支平衡：真菌线粒体中RNA剪接的一种模型。
Nature. 1982 Dec 23;300(5894):719-24. doi: 10.1038/300719a0.
6
Cloning and expression of the gene for bacteriophage T7 RNA polymerase.噬菌体T7 RNA聚合酶基因的克隆与表达。
Proc Natl Acad Sci U S A. 1984 Apr;81(7):2035-9. doi: 10.1073/pnas.81.7.2035.
7
Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena.自我剪接RNA：嗜热四膜虫核糖体RNA间隔序列的自动切除与自动环化
Cell. 1982 Nov;31(1):147-57. doi: 10.1016/0092-8674(82)90414-7.
8
The intervening sequence of the ribosomal RNA gene is highly conserved between two Tetrahymena species.两种四膜虫物种的核糖体RNA基因的间隔序列高度保守。
Nucleic Acids Res. 1982 May 11;10(9):2809-22. doi: 10.1093/nar/10.9.2809.
9
A labile phosphodiester bond at the ligation junction in a circular intervening sequence RNA.环状间隔序列RNA连接位点处的不稳定磷酸二酯键。
Science. 1984 May 11;224(4649):574-8. doi: 10.1126/science.6200938.
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The RNA moiety of ribonuclease P is the catalytic subunit of the enzyme.核糖核酸酶P的RNA部分是该酶的催化亚基。
Cell. 1983 Dec;35(3 Pt 2):849-57. doi: 10.1016/0092-8674(83)90117-4.