South China Agricultural University, Guangdong, Guangzhou, China.
Vaccine. 2011 Nov 3;29(47):8677-82. doi: 10.1016/j.vaccine.2011.08.118. Epub 2011 Sep 21.
The capsid structural protein encoded by the gene ORF2 of Porcine Circovirus Type 2 is critical for eliciting the broad protective immunity. We constructed and characterized the recombinant adenovirus encoding the capsid and porcine IFN gamma, designated as rAd-ORF2-IFN-γ. The construct was further confirmed by the enzyme digestion, PCR, sequencing and transfection. The humoural immunity to the capsid protein, induced by the recombinant in the mice, was tested by ELISA. Notably, this recombinant induced a much better ORF2-specific antibody response than that of rAd-ORF2 alone or the adenovirus itself. Clearly, the porcine IFN gamma could strongly enhance the immunogenicity of ORF2. The results showed that the recombinant adenovirus might be an attractive candidate vaccine for preventing the disease associated with PCV2 infection.
猪圆环病毒 2 型基因 ORF2 编码的衣壳结构蛋白对于激发广泛的保护免疫至关重要。我们构建并鉴定了编码衣壳和猪干扰素γ的重组腺病毒，命名为 rAd-ORF2-IFN-γ。该构建物通过酶切、PCR、测序和转染进一步得到证实。通过 ELISA 检测重组在小鼠中诱导的针对衣壳蛋白的体液免疫。值得注意的是，与 rAd-ORF2 单独或腺病毒本身相比，该重组诱导了更好的 ORF2 特异性抗体反应。显然，猪干扰素γ可以强烈增强 ORF2 的免疫原性。结果表明，该重组腺病毒可能是预防与 PCV2 感染相关疾病的有吸引力的候选疫苗。
