Key Laboratory of Mammalian Reproductive Biology and Biotechnology Ministry of Education, Inner Mongolia University, Inner Mongolia, Hohhot 010021, China.
Res Vet Sci. 2012 Aug;93(1):404-11. doi: 10.1016/j.rvsc.2011.08.014. Epub 2011 Sep 25.
A goat adipose-derived stem cell (ADSC) line was established and compared to a rat line. Goat ADSC cells had normal diploidy after subculture. Proliferation of goat ADSCs was faster than rat cells in the same conditions. Both rat and goat ADSCs stained positively for vimentin, CD49d, CD44 and CD13, but stained negatively for CD34 and CD106. Bone nodules were apparent, and alizarin staining was positive after osteogenic induction. Cells expressing osteocalcin were positive by alkaline phosphatase (ALP) staining. After osteogenic induction, ossification nodules of goat ADSCs were larger than in rats, with dense ALP staining. Adipogenic induction resulting in lipid droplets and peroxisome proliferator-activated receptor (PPARγ2) expression were observed. Cartilage lacunae were formed and COL2A1 was expressed. More cartilage lacunae with better morphology were seen following differentiation of goat ADSC's using the hang-drop method. For goat ADSCs, results with both adherent-induced and hanging-drop induced cultures were better than for three-dimensional cultures.
建立了山羊脂肪干细胞（ADSC）系，并与大鼠细胞系进行了比较。山羊 ADSC 细胞在传代后具有正常的二倍体。在相同条件下，山羊 ADSC 的增殖速度快于大鼠细胞。大鼠和山羊 ADSC 均对波形蛋白、CD49d、CD44 和 CD13 呈阳性染色，而对 CD34 和 CD106 呈阴性染色。成骨诱导后出现明显的骨结节，茜素红染色阳性。碱性磷酸酶（ALP）染色阳性的细胞表达骨钙素。成骨诱导后，山羊 ADSC 的骨化结节大于大鼠，ALP 染色致密。诱导脂肪生成后，观察到脂滴和过氧化物酶体增殖物激活受体（PPARγ2）的表达。形成软骨陷窝并表达 COL2A1。使用悬滴法对山羊 ADSC 进行分化后，观察到更多形态更好的软骨陷窝。对于山羊 ADSC，贴壁诱导和悬滴诱导培养的结果均优于三维培养。
