[作为人胎盘碱性磷酸酶产生者的M-HeLa细胞的免疫化学研究]
[An immunochemical study of M-HeLa cells as producers of human placental alkaline phosphatase].
作者信息
Gudima S O, Liakhov V V, Sokolov A V, Terekhov O P, Serov S M, Sukhikh G T, Vengerov Iu Iu
出版信息
Biull Eksp Biol Med. 1990 Mar;109(3):285-7.
While analyzing M-HeLa cells by IFA technique secretory and membrane-bound forms of human placental alkaline phosphatase (HPAP) were detected. Activity of secretory HPAP increased if cell density and incubation time were increased too. After short influence of heat shock (15 min at 42 degrees C) activity of secretory HPAP increased for 45% and intracellular HPAP 3 for 37%. It is proposed that HPAP take part in organization of first response to heat shock and support cellular thermotolerance.
在用免疫荧光分析技术分析M-HeLa细胞时，检测到了人胎盘碱性磷酸酶（HPAP）的分泌形式和膜结合形式。如果细胞密度和孵育时间增加，分泌型HPAP的活性也会增加。在热休克短时间作用（42℃下15分钟）后，分泌型HPAP的活性增加了45%，细胞内HPAP 3增加了37%。有人提出，HPAP参与了对热休克的初始反应的组织，并支持细胞耐热性。
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