State Key Laboratory of Genetic Engineering, Institute of Genetics, School of Life Science, Fudan University, Shanghai 200433, China.
Biochem Biophys Res Commun. 2011 Oct 14;414(1):181-5. doi: 10.1016/j.bbrc.2011.09.053. Epub 2011 Sep 17.
TGF-β (transforming growth factor-β), a pleiotropic cytokine that regulates diverse cellular processes, has been suggested to play critical roles in cell proliferation, migration, and carcinogenesis. Here we found a novel E3 ubiquitin ligase RLIM which can directly bind to Smurf2, enhancing TGF-β responsiveness in osteosarcoma U2OS cells. We constructed a U2OS cell line stably over-expressing RLIM and demonstrated that RLIM promoted TGF-β-driven migration of U2OS cells as tested by wound healing assay. Our results indicated that RLIM is an important positive regulator in TGF-β signaling pathway and cell migration.
TGF-β（转化生长因子-β）是一种多效细胞因子，调节多种细胞过程，被认为在细胞增殖、迁移和癌变中发挥关键作用。在这里，我们发现了一种新的 E3 泛素连接酶 RLIM，它可以直接与 Smurf2 结合，增强骨肉瘤 U2OS 细胞对 TGF-β的反应性。我们构建了一个稳定过表达 RLIM 的 U2OS 细胞系，并通过划痕实验证明 RLIM 促进了 TGF-β驱动的 U2OS 细胞迁移。我们的结果表明，RLIM 是 TGF-β信号通路和细胞迁移的重要正调控因子。
