Pesticide Toxicology Division, CSIR-Indian Institute of Toxicology Research, PO Box No. 80, Mahatma Gandhi Marg, Lucknow 226 001, India.
Food Chem Toxicol. 2011 Dec;49(12):3086-9. doi: 10.1016/j.fct.2011.09.009. Epub 2011 Sep 16.
Technical imidacloprid was evaluated for its effect on ovarian morphology, hormones and antioxidant enzymes in female rats after 90 days oral exposure. Luteinizing hormone (LH), follicle stimulating hormone (FSH) and progesterone levels were estimated in serum of rats and activity of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) and level of reduced glutathione (GSH) and lipid peroxidation (LPO) were estimated in ovary after oral administration of imidacloprid (5, 10, and 20mg/kg/day) for 90 days. Decreased ovarian weight together with significant patho-morphological changes in follicles, antral follicles and atretic follicles were observed at 20mg/kg/day. Imidacloprid at 5 and 10mg/kg/day has not produced any significant changes in ovarian morphology, hormones and antioxidant status of ovary. However 20mg/kg/day dose has produced significant alterations in the levels of LH, FSH and progesterone. Similarly significant changes in SOD, CAT, GPx, GSH, and LPO were observed at 20mg/kg/day dose level. Therefore, it is concluded that imidacloprid at 20mg/kg/day dose level has produced significant toxicological impact on ovary of female rats as evident by pathomorphological changes, hormonal imbalance and generating oxidative stress and can be considered primarily as Lowest Observed Effect Level (LOEL) for chronic study.
在 90 天的口服暴露后，技术吡虫啉被评估对雌性大鼠卵巢形态、激素和抗氧化酶的影响。在口服吡虫啉（5、10 和 20mg/kg/天）90 天后，估计大鼠血清中的促黄体生成激素（LH）、卵泡刺激素（FSH）和孕酮水平，并估计卵巢中超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GPx）和还原型谷胱甘肽（GSH）的活性以及脂质过氧化（LPO）水平。在 20mg/kg/天剂量下，卵巢重量降低，同时观察到卵泡、窦卵泡和闭锁卵泡的显著病理形态变化。吡虫啉在 5 和 10mg/kg/天剂量下未对卵巢形态、激素和卵巢抗氧化状态产生任何显著变化。然而，20mg/kg/天剂量导致 LH、FSH 和孕酮水平发生显著变化。在 20mg/kg/天剂量水平下，SOD、CAT、GPx、GSH 和 LPO 也观察到显著变化。因此，结论是，吡虫啉在 20mg/kg/天剂量水平下对雌性大鼠卵巢产生了显著的毒理学影响，表现为病理形态变化、激素失衡、产生氧化应激，可被视为慢性研究的最低观察效应水平（LOEL）。
