Department of Restorative Dentistry, Faculty of Dentistry, King Abdulaziz University, Jeddah, Saudi Arabia.
Department of Oral Biology, Cairo University School of Dentistry, Cairo, Egypt.
Ann Med. 2023;55(2):2258917. doi: 10.1080/07853890.2023.2258917. Epub 2023 Sep 28.
Magnesium oxide nanoparticles (MgO NPs) have a variety of applications that have contributed to their elevated popularity, however, the safety and toxic effects on humans are also of concern with these increased applications. There is insufficient data regarding the effect of MgO NPs on reproductive organs, which are crucial aspects to the body's vital physiological functions. The present study was undertaken in male and female rats to assess the reproductive toxicological potential of two doses (low versus high) of MgO NPs on testicular and ovarian tissues. The toxicity was evaluated using histological, hormonal, and oxidative parameters.
In this work, magnesium oxide nanoparticles (MgO NPs) were synthesized by the sol-gel route and were characterized by X ray diffraction analysis (XRD) and Fourier transform infra-red spectroscopy (FTIR). Forty-eight adult Wister albino rats were used in this experiment which were divided into groups of male and female, and then further into control, low dose MgO NPs, and high dose MgO NPs. The low dose used was 131.5 mg/kg b.w. (1/10 LD50) while the high dose used was 263 mg/kg b.w. (1/5 LD50). All doses were given orally by gastric tube. After 4 weeks, blood samples were collected to investigate the level of sex hormones and both ovarian and testicular tissues were examined for variable oxidative parameters and histopathological changes by light microscopy.
The obtained findings showed that high dose of MgO NPs produced considerable changes in sex hormones and stress parameters in both male and female rats in comparison to the low dose and control groups. Histomorphometric analysis demonstrated the presence of histopathological alterations in the testicular and ovarian tissues.
The results of this study showed dose-dependent adverse effects of MgO NPs on the testis and ovary both functionally and histopathologically as compared to the control rats.
氧化镁纳米粒子（MgO NPs）具有多种应用，这使得它们的应用越来越广泛，然而，随着这些应用的增加，人们对其安全性和对人类的毒性作用也越来越关注。关于 MgO NPs 对生殖器官的影响的数据还不够充分，而生殖器官是人体重要生理功能的关键方面。本研究在雄性和雌性大鼠中进行，以评估两种剂量（低剂量与高剂量）的 MgO NPs 对睾丸和卵巢组织的生殖毒性作用。使用组织学、激素和氧化参数评估毒性。
在这项工作中，通过溶胶-凝胶法合成了氧化镁纳米粒子（MgO NPs），并通过 X 射线衍射分析（XRD）和傅里叶变换红外光谱（FTIR）进行了表征。本实验使用了 48 只成年 Wister 白化大鼠，将其分为雄性和雌性两组，然后进一步分为对照组、低剂量 MgO NPs 组和高剂量 MgO NPs 组。低剂量组为 131.5mg/kg b.w.（1/10 LD50），高剂量组为 263mg/kg b.w.（1/5 LD50）。所有剂量均通过胃管口服给药。4 周后，采集血液样本以研究性激素水平，通过光镜检查卵巢和睾丸组织的各种氧化参数和组织病理学变化。
研究结果表明，与低剂量组和对照组相比，高剂量的 MgO NPs 对雄性和雌性大鼠的性激素和应激参数产生了相当大的变化。组织形态计量学分析表明，睾丸和卵巢组织存在组织病理学改变。
与对照组大鼠相比，本研究结果表明，MgO NPs 对睾丸和卵巢具有剂量依赖性的不良影响，无论是在功能上还是在组织病理学上。
