Institute of Food Engineering, Shanghai Normal University, 100 Guilin Rd, Shanghai 200234, PR China.
Int J Biol Macromol. 2011 Dec 1;49(5):1143-51. doi: 10.1016/j.ijbiomac.2011.09.013. Epub 2011 Sep 19.
The chemical compositions and bioactivities of crude tea polysaccharides (TPS) from the out-of-date tea leaves (beyond their useful date), namely Xihu Longjing (XTPS), Anxi Tieguanyin (TTPS), Chawentianxia (CTPS) and Huizhoulvcha (HTPS), in market were investigated. These TPS showed similar neutral sugar content and different distribution of molecular weight (1-800 kD). These crude TPS were mainly composed of rhamnose, arabinose, galactose, glucose, xylose, mannose, and galacturonic acid. IR spectra confirmed that these crude TPS were composed of polysaccharide, protein and uronic acids. These TPS showed similar DPPH scavenging activity and exhibited lower DPPH scavenging activities than Vc within 25-200 μg/mL. However, these TPS with higher concentrations (200-400 μg/mL) showed similar DPPH scavenging activity with Vc. HTPS exhibited significant higher superoxide anion scavenging activity than others TPS and gallic acid. XTPS showed significant higher inhibitory effects on α-glucosidase and α-amylase with inhibitory percentages of 64.35% and 82.24% than others TPS. TTPS, XTPS, and HTPS exhibited similar inhibition ability on α-d-glucosidase and α-amylase. The overdue tea leaves can be a resource of tea polysaccharides as function food.
研究了市场上过期茶叶(超过保质期)制成的粗茶多糖(TPS)的化学成分和生物活性,包括西湖龙井(XTPS)、安溪铁观音(TTPS)、岔尾天香(CTPS)和惠洲绿茶(HTPS)。这些 TPS 具有相似的中性糖含量和不同的分子量分布(1-800 kD)。这些粗 TPS 主要由鼠李糖、阿拉伯糖、半乳糖、葡萄糖、木糖、甘露糖和半乳糖醛酸组成。IR 光谱证实这些粗 TPS 由多糖、蛋白质和糖醛酸组成。这些 TPS 具有相似的 DPPH 清除活性,在 25-200 μg/mL 范围内的 DPPH 清除活性低于 Vc。然而,这些 TPS 浓度较高(200-400 μg/mL)时,其 DPPH 清除活性与 Vc 相似。HTPS 对超氧阴离子的清除活性明显高于其他 TPS 和没食子酸。XTPS 对α-葡萄糖苷酶和α-淀粉酶的抑制作用明显高于其他 TPS,抑制率分别为 64.35%和 82.24%。TTPS、XTPS 和 HTPS 对α-葡萄糖苷酶和α-淀粉酶的抑制能力相似。过期茶叶可以作为功能食品的茶多糖资源。