Institute of Food Engineering, College of Life & Environment Science, Shanghai Normal University, 100 Guilin Road, Shanghai 200234, PR China.
Int J Biol Macromol. 2013 Nov;62:714-9. doi: 10.1016/j.ijbiomac.2013.10.006. Epub 2013 Oct 10.
Four polysaccharides (TPS-F, TPS-V, TPS-S and TPS-M) were obtained from tea (Camellia sinensis) leaves by freeze-drying, vacuum-drying, spray-drying and microwave-vacuum drying, respectively. Their chemical composition and biological properties were comparatively studied. The results showed these TPS were similar in IR, UV absorption and distribution of molecular weight. However, they showed significant differences (P<0.05) in yields of crude polysaccharides and contents of protein and total polyphenols. Furthermore, morphological analysis showed their surface differed from each other in size and shape when viewed by SEM, from which surface of TPS-F was rough and porous, surface of TPS-S was evenly particles, and surfaces of the other two were similar to anomalistic bricks. In addition, the bioactivity of them was also evaluated. It was found TPS-F exhibited relatively better ability on metal chelating and superoxide radicals scavenging assays than others, and TPS-V exhibited higher ability on α-glycosidase and α-amylase inhibition assays than others with inhibitory percentages of 82.75% and 92.8%.
四种多糖（TPS-F、TPS-V、TPS-S 和 TPS-M）分别通过冷冻干燥、真空干燥、喷雾干燥和微波真空干燥从茶叶（Camellia sinensis）中提取得到。对它们的化学成分和生物活性进行了比较研究。结果表明，这些 TPS 在红外（IR）、紫外吸收和分子量分布方面相似。然而，它们在粗多糖的产率、蛋白质和总多酚的含量方面存在显著差异（P<0.05）。此外，形态分析表明，通过扫描电子显微镜（SEM）观察，它们的表面在大小和形状上存在差异，TPS-F 的表面粗糙多孔，TPS-S 的表面均匀颗粒状，而另外两种的表面则类似于不规则的砖块。此外，还对它们的生物活性进行了评估。结果发现，TPS-F 在金属螯合和超氧自由基清除测定中表现出相对较好的能力，而 TPS-V 在α-葡萄糖苷酶和α-淀粉酶抑制测定中表现出比其他两种更高的能力，抑制率分别为 82.75%和 92.8%。
