Atopkin Dmitri M
Institute of Biology and Soil Science, Far Eastern Branch of the Russian Academy of Sciences, Vladivostok, Russia.
Parasitol Int. 2011 Dec;60(4):541-3. doi: 10.1016/j.parint.2011.09.005. Epub 2011 Sep 17.
In the present study, we successfully amplified the 28S ribosomal DNA of 3 species of the Psilotrema genus (Psilotrema oschmarini Besprozvannykh, 2007, P. acutirostris Oschmarin, 1963 and P. simillimum Muhling, 1989) from the Russian Far East. The genetic divergence between these species was estimated, and the phylogenetic relationships of the Psilotrema genus and the Psilostomatidae family were inferred. The resulting data support the independent taxonomical status of P. oschmarini as a valid species in the Russian Far East. In spite of the considerable morphological differences between P. simillimum and P. acutirostris, these species are genetically very close to each other. Genetic distances, calculated from the pairwise comparison of two obtained clusters using transversions, show that Psilostomatidae is the most divergent family. Phylogenetic analysis revealed two clusters, which corresponds to family Psilostomatidae (basal cluster) and other Echinostomatoidea species.
在本研究中，我们成功扩增了来自俄罗斯远东地区的3种裸口吸虫属（Psilotrema）物种（2007年的奥斯氏裸口吸虫Psilotrema oschmarini Besprozvannykh、1963年的尖吻裸口吸虫P. acutirostris Oschmarin和1989年的相似裸口吸虫P. simillimum Muhling）的28S核糖体DNA。估计了这些物种之间的遗传差异，并推断了裸口吸虫属与裸口科的系统发育关系。所得数据支持奥斯氏裸口吸虫在俄罗斯远东地区作为一个有效物种的独立分类地位。尽管相似裸口吸虫和尖吻裸口吸虫之间存在相当大的形态差异，但这些物种在基因上彼此非常接近。通过使用颠换对两个所得聚类进行成对比较计算的遗传距离表明，裸口科是差异最大的科。系统发育分析揭示了两个聚类，分别对应裸口科（基部聚类）和其他棘口超科物种。
