Li Jinrong, Yan Jianqun, Chen Ke, Wang Qian, Zhao Xiaolin, Zhang Yuan
Department of Physiology and Pathophysiology, Xi'an Jiaotong University School of Medicine, Xi'an 710061, China.
Zhong Nan Da Xue Xue Bao Yi Xue Ban. 2011 Sep;36(9):823-9. doi: 10.3969/j.issn.1672-7347.2011.09.002.
To investigate the effect of high-fat (HF) diet on the body weight and the mRNA expression of melanin concentrating hormone receptor 1 (MCHR1) and leptin receptor (OB-Rb) in the adipose tissue in rats, the two important and opposite factors in regulating the body weight.
Post-weaning rats were divided into 3 groups: the NC group were fed a normal-chow diet (NC) (13% calories from fat), the HF group with a HF-diet (47% calories from fat) and the PHF group pair-fed a HF-diet (47% calories from fat). At the end of 8th week, the gained bodyweight, the plasma melanin concentrating hormone (MCH) and leptin, and the expression levels of MCHR1 and OB-Rb in the adipose tissue were measured.
Both the HF-diet and pair-fed HF-diet enhanced the body weight (P<0.01), plasma MCH (P<0.01) and leptin concentrations (P<0.05). In the adipose tissue, HF-diet resulted in significant increase in MCHR1 (PHF group,P<0.05) and decrease in OB-Rb mRNA levels (HF group,P<0.01; PHF group,P<0.05). No statistical difference was found between the HF group and the PHF group in terms of the aforementioned data (P>0.05).
Chronic intake of iso-caloric HF-diet and ad libitum HF-diet obviously results in increase in the body weight, serum leptin, and MCH concentration. Diet-induced obesity and related metabolic disorders are possibly correlated with up-regulated expression of MCHR1 and down-regulated expression of OB-Rb in the adipose tissue.
研究高脂（HF）饮食对大鼠体重以及脂肪组织中黑色素浓缩激素受体1（MCHR1）和瘦素受体（OB-Rb）mRNA表达的影响，这两个因素在调节体重方面作用相反且至关重要。
将断奶后的大鼠分为3组：正常对照组（NC组）喂食正常饲料（NC）（脂肪提供13%的热量），高脂组喂食高脂饲料（HF）（脂肪提供47%的热量），配对喂食高脂组（PHF组）按高脂组的进食量配对喂食高脂饲料（脂肪提供47%的热量）。在第8周结束时，测量体重增加量、血浆黑色素浓缩激素（MCH）和瘦素水平，以及脂肪组织中MCHR1和OB-Rb的表达水平。
高脂饮食组和配对喂食高脂组均使体重增加（P<0.01）、血浆MCH升高（P<0.01）以及瘦素浓度增加（P<0.05）。在脂肪组织中，高脂饮食导致MCHR1显著增加（PHF组，P<0.05），OB-Rb mRNA水平降低（HF组，P<0.01；PHF组，P<0.05）。上述数据在高脂组和配对喂食高脂组之间未发现统计学差异（P>0.05）。
长期摄入等热量的高脂饮食和随意进食高脂饮食均明显导致体重增加、血清瘦素和MCH浓度升高。饮食诱导的肥胖及相关代谢紊乱可能与脂肪组织中MCHR1表达上调和OB-Rb表达下调有关。
