Furuhata Katsunori, Banzai Azusa U, Kawakami Yasushi, Ishizaki Naoto, Yoshida Yoshihiro, Goto Keiichi, Fukuyama Masafumi
School of Life and Environmental Science, Azabu University, Kanagawa, Japan.
Biocontrol Sci. 2011 Sep;16(3):103-7. doi: 10.4265/bio.16.103.
For microbial ecological analysis, 14 strains of Methylobacterium aquaticum isolated from water samples were subjected to clustering analysis on the basis of ribotyping and RAPD-PCR tests. The ribopatterns after digestion with EcoRI obtained from 14 strains of M. aquaticum were used to divide the strains into two groups (Groups I and II) with a similarity of 55%. From the analysis of RAPD patterns using primer 208, the 14 strains were divided into 3 groups (A-C) based on a homology of 45% or greater, and from that using primer 272, there were 4 groups (A-D) based on a homology of 50% or greater. The chlorine resistance (99.9% CT values) of these isolates was also experimentally confirmed, and we attempted to define the connection between chlorine resistance and the geno-cluster. The average CT value of group I was 0.89 mg•min/l and the average of group II was 0.69 mg•min/l. No remarkable differences in the CT values for the groups were found.
为进行微生物生态分析，对从水样中分离出的14株水生甲基杆菌进行了基于核糖体分型和随机扩增多态性DNA聚合酶链反应（RAPD-PCR）测试的聚类分析。用EcoRI消化14株水生甲基杆菌后得到的核糖体图谱用于将这些菌株分为两组（I组和II组），相似性为55%。通过使用引物208对RAPD图谱进行分析，基于45%或更高的同源性将14株菌株分为3组（A - C组），而使用引物272进行分析时，基于50%或更高的同源性有4组（A - D组）。还通过实验证实了这些分离株的耐氯性（99.9% 杀菌浓度时间值），并且我们试图确定耐氯性与基因簇之间的联系。I组的平均杀菌浓度时间值为0.89毫克·分钟/升，II组的平均值为0.69毫克·分钟/升。未发现各组之间杀菌浓度时间值有显著差异。
