Furuhata Katsunori, Ishizaki Naoto, Umekawa Nao, Nishizima Miyuki, Fukuyama Masafumi
School of Life and Environmental Science, Azabu University.
Biocontrol Sci. 2014;19(1):33-8. doi: 10.4265/bio.19.33.
For a microbial ecological analysis, 20 strains of Legionella pneumophila isolated from both unchlorinated Noyu (unattended natural hot spring) samples and chlorinated hot spring bath water samples collected throughout Japan were subjected to a clustering analysis on the basis of a Pulsed-Field Gel Electrophoresis (PFGE) pattern analysis. The PFGE patterns obtained from 19 strains of L. pneumophila after digestion with SfiI were used to divide the strains into two groups (Groups A and B), although the similarity level was very low among the groups. Group A consisted of 8 strains, and all of these strains were isolated from hot spring bath water samples. Group B consisted of 11 strains, and all but two of these strains were isolated from Noyu samples. The chlorine resistance (99.9% CT values) of these isolates was experimentally confirmed, and we attempted to define the relationship between chlorine resistance and the geno-cluster. The average CT value of group A (8 strains from hot spring bath water) was 0.49 mg･min/l and the average of group B (9 strains from Noyu samples) was 0.29 mg･min/l. No remarkable differences in the CT values for the groups were found. A chlorine-sensitive Noyu strain (0.14 mg･min/l) and a chlorine-resistant strain (0.62 mg･min/l) from hot spring bath water were then compared to identify any differences in their lipid composition. There was no notable difference in the ratio of saturated to unsaturated fatty acids between the chlorine-sensitive and chlorine-resistant bacteria. However, the chlorine-sensitive and chlorine-resistant bacteria demonstrated differences in the relative percentages of cell wall and cell membrane fatty acids.
为进行微生物生态分析，从日本各地采集的未氯化的野汤（无人值守的天然温泉）样本和氯化温泉浴水样本中分离出20株嗜肺军团菌，基于脉冲场凝胶电泳（PFGE）图谱分析对其进行聚类分析。用SfiI酶切后从19株嗜肺军团菌获得的PFGE图谱用于将菌株分为两组（A组和B组），尽管两组之间的相似性水平非常低。A组由8株菌株组成，所有这些菌株均从温泉浴水样本中分离得到。B组由11株菌株组成，其中除两株外的所有菌株均从野汤样本中分离得到。通过实验证实了这些分离株的耐氯性（99.9% CT值），并且我们试图确定耐氯性与基因簇之间的关系。A组（来自温泉浴水的8株菌株）的平均CT值为0.49 mg･min/l，B组（来自野汤样本的9株菌株）的平均CT值为0.29 mg･min/l。两组的CT值未发现显著差异。然后比较了一株对氯敏感的野汤菌株（0.14 mg･min/l）和一株来自温泉浴水的耐氯菌株（0.62 mg･min/l），以确定它们脂质组成的差异。对氯敏感和耐氯细菌之间饱和脂肪酸与不饱和脂肪酸的比例没有显著差异。然而，对氯敏感和耐氯细菌在细胞壁和细胞膜脂肪酸的相对百分比上表现出差异。
