Institute of Hydrochemistry, Technische Universität München, Marchioninistrasse 17, 81377 Munich, Germany.
Anal Bioanal Chem. 2012 Mar;402(8):2663-7. doi: 10.1007/s00216-011-5398-0. Epub 2011 Sep 23.
We present an immunoassay microarray flow-through system for the surface-enhanced Raman scattering (SERS) analysis of bacteria. The system has been constructed to support and automatize the nondestructive in situ analysis of different microorganisms in aqueous environment. After the immobilization of the desired antibodies to an activated PEG-coated surface, the chip is placed into the flow cell which is then flushed with the contaminated sample. Finally, colloidal metal nanoparticles are added and the cells are detected label-free by SERS. Here, we introduce the successful imaging of single microorganisms in the flow cell as well as the quantification of microorganisms in water by SERS mapping with a linear range between 4.3 × 10(3) to 4.3 × 10(5) cells/mL. The method has potential for routine application, e.g. for drinking water control.
我们提出了一种用于表面增强拉曼散射（SERS）分析细菌的免疫分析微流控系统。该系统旨在支持和自动化在水相环境中对不同微生物进行非破坏性原位分析。在将所需抗体固定到激活的聚乙二醇（PEG）涂层表面后，将芯片放入流动池，然后用污染样品冲洗。最后，加入胶体金属纳米粒子，并通过 SERS 进行无标记的细胞检测。在这里，我们成功地在流动池中对单个微生物进行成像，并通过 SERS 映射对水中的微生物进行定量，线性范围在 4.3×10(3)到 4.3×10(5)细胞/mL 之间。该方法具有常规应用的潜力，例如用于饮用水控制。
