International Iberian Nanotechnology Laboratory (INL), Avda Mestre José Veiga, 4715-310 Braga, Portugal.
Biosensors (Basel). 2018 Jun 29;8(3):62. doi: 10.3390/bios8030062.
Raman scattering and surface-enhanced Raman scattering (SERS) spectroscopy have demonstrated their potential as ultrasensitive detection techniques in the past decades. Specifically, and as a result of the flourishing of nanotechnology, SERS is nowadays one of the most powerful sensing techniques, not only because of the low detection limits that it can achieve, but also for the structural information that it offers and its capability of multiplexing. Similarly, microfluidics technology is having an increased presence not only in fundamental research, but also in the industry. The latter is because of the intrinsic characteristics of microfluidics, being automation, high-throughput, and miniaturization. However, despite miniaturization being an advantage, it comes together with the need to use ultrasensitive techniques for the interrogation of events happening in extremely small volumes. The combination of SERS with microfluidics can overcome bottlenecks present in both technologies. As a consequence, the integration of Raman and SERS in microfluidics is being investigated for the label-free biosensing of relevant research challenges.
在过去的几十年中，拉曼散射和表面增强拉曼散射（SERS）光谱已经证明了它们作为超灵敏检测技术的潜力。具体来说，由于纳米技术的蓬勃发展，SERS 现在是最强大的传感技术之一，不仅因为它可以实现低检测限，还因为它提供的结构信息及其多路复用能力。同样，微流控技术不仅在基础研究中越来越受到重视，而且在工业中也越来越受到重视。后者是由于微流控技术的固有特性，即自动化、高通量和小型化。然而，尽管小型化是一个优势，但它需要使用超灵敏技术来检测在极小体积中发生的事件。将 SERS 与微流控技术相结合可以克服这两种技术中存在的瓶颈。因此，正在研究将拉曼和 SERS 集成到微流控中，以实现对相关研究挑战的无标记生物传感。
