Johnston R F, Pickett S C, Barker D L
Molecular Dynamics, Sunnyvale, CA 94086.
Electrophoresis. 1990 May;11(5):355-60. doi: 10.1002/elps.1150110503.
We describe the application of photostimulable storage phosphor imaging plates to autoradiography of samples labeled with 32P, 14C and 35S. Imaging plates can detect a 32P-labeled sample at an exposure level of 1 disintegration/mm2, and 14C and 35S at 25 disintegrations/mm2. Compared to X-ray film, imaging plates offer increased sensitivity of 15- to 250-fold for 32P and 20- to 100-fold for 14C and 35S. Resolution is in the 0.3 mm range and is therefore suitable for any gel or blot application.
我们描述了光激发存储磷光成像板在32P、14C和35S标记样品放射自显影中的应用。成像板能够检测到暴露水平为1次衰变/mm2的32P标记样品，以及暴露水平为25次衰变/mm2的14C和35S标记样品。与X射线胶片相比，成像板对32P的灵敏度提高了15至250倍，对14C和35S的灵敏度提高了20至100倍。分辨率在0.3mm范围内，因此适用于任何凝胶或印迹应用。
