DNA重组与修复研究指南：DNA修复途径的细胞分析
Guidelines for DNA recombination and repair studies: Cellular assays of DNA repair pathways.
作者信息
Klein Hannah L, Bačinskaja Giedrė, Che Jun, Cheblal Anais, Elango Rajula, Epshtein Anastasiya, Fitzgerald Devon M, Gómez-González Belén, Khan Sharik R, Kumar Sandeep, Leland Bryan A, Marie Léa, Mei Qian, Miné-Hattab Judith, Piotrowska Alicja, Polleys Erica J, Putnam Christopher D, Radchenko Elina A, Saada Anissia Ait, Sakofsky Cynthia J, Shim Eun Yong, Stracy Mathew, Xia Jun, Yan Zhenxin, Yin Yi, Aguilera Andrés, Argueso Juan Lucas, Freudenreich Catherine H, Gasser Susan M, Gordenin Dmitry A, Haber James E, Ira Grzegorz, Jinks-Robertson Sue, King Megan C, Kolodner Richard D, Kuzminov Andrei, Lambert Sarah Ae, Lee Sang Eun, Miller Kyle M, Mirkin Sergei M, Petes Thomas D, Rosenberg Susan M, Rothstein Rodney, Symington Lorraine S, Zawadzki Pawel, Kim Nayun, Lisby Michael, Malkova Anna
机构信息
Department of Biochemistry and Molecular Pharmacology, New York University School of Medicine, New York, NY, USA.
Department of Biology, University of Copenhagen, DK-2200 Copenhagen N, Denmark.
出版信息
Microb Cell. 2019 Jan 7;6(1):1-64. doi: 10.15698/mic2019.01.664.
Understanding the plasticity of genomes has been greatly aided by assays for recombination, repair and mutagenesis. These assays have been developed in microbial systems that provide the advantages of genetic and molecular reporters that can readily be manipulated. Cellular assays comprise genetic, molecular, and cytological reporters. The assays are powerful tools but each comes with its particular advantages and limitations. Here the most commonly used assays are reviewed, discussed, and presented as the guidelines for future studies.
用于重组、修复和诱变的检测方法极大地促进了对基因组可塑性的理解。这些检测方法是在微生物系统中开发的，微生物系统具有易于操作的遗传和分子报告基因的优势。细胞检测包括遗传、分子和细胞学报告基因。这些检测方法是强大的工具，但每种方法都有其独特的优点和局限性。本文对最常用的检测方法进行了综述、讨论，并作为未来研究的指南呈现。
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