Faculdade de Ciências Agrárias e Veterinárias de Jaboticabal, UNESP, São Paulo, Brazil.
Mol Biol Rep. 2012 Apr;39(4):4837-40. doi: 10.1007/s11033-011-1277-1. Epub 2011 Sep 27.
We describe the isolation and characterization of ten microsatellite loci from the red-winged tinamou (Rhynchotus rufescens) and also evaluated the cross-amplification of these loci and other ten loci previously developed for the great tinamou (Tinamus major) in other tinamous. Genetic variability was assessed using 24 individuals. Six loci were polymorphic with moderate to high number of alleles per locus (2-12 alleles) and showed expected heterozygosity (HE) ranging from 0.267 to 0.860. All loci conformed to the Hardy-Weinberg expectation and linkage disequilibrium was not significant for any pair of loci. This battery of polymorphic loci showed high paternity exclusion probability (0.986) and low genetic identity probability (4.95 × 10(-5)), proving to be helpful for parentage tests and population analyses in the red-winged tinamou. The cross-amplification was moderate where of the 160 locus/taxon combinations, 46 (28.75%) successfully amplified.
我们描述了红翼凤头鸊鷉（Rhynchotus rufescens）的十个微卫星位点的分离和特征，并评估了这些位点在其他凤头麦鸡中的交叉扩增情况，这些位点和其他十个位点之前是为大凤头麦鸡（Tinamus major）开发的。利用 24 个个体评估了遗传变异性。六个位点具有中等至高度多态性，每个位点的等位基因数量（2-12 个等位基因），表现出预期的杂合度（HE）范围为 0.267 至 0.860。所有位点均符合哈迪-温伯格期望，且没有任何一对位点存在连锁不平衡。这组多态性位点具有高的亲权排除概率（0.986）和低的遗传身份概率（4.95×10（-5）），证明对红翼凤头鸊鷉的亲子关系测试和种群分析很有帮助。交叉扩增适中，在 160 个位点/分类群组合中，成功扩增了 46 个（28.75%）。
