普通渡鸦（Corvus corax）微卫星位点的开发与特征分析及在其他鸦科鸟类中的跨物种扩增
Development and characterization of microsatellite loci for common raven (Corvus corax) and cross species amplification in other Corvidae.
作者信息
Pruett Christin L, Wan Leping, Li Tianyu, Spern Cory, Lance Stacey L, Glenn Travis, Faircloth Brant, Winker Kevin
机构信息
Department of Biological Sciences, Florida Institute of Technology, Melbourne, FL, 32901, USA.
Savannah River Ecology Laboratory, University of Georgia, Aiken, SC, 29802, USA.
出版信息
BMC Res Notes. 2015 Nov 6;8:655. doi: 10.1186/s13104-015-1643-5.
BACKGROUND
A priority for conservation is the identification of endemic populations. We developed microsatellite markers for common raven (Corvus corax), a bird species with a Holarctic distribution, to identify and assess endemic populations in Alaska.
RESULTS
From a total of 50 microsatellite loci, we isolated and characterized 15 loci. Eight of these loci were polymorphic and readily scoreable. Eighteen to 20 common ravens from Fairbanks, Alaska were genotyped showing the following variability: 3-8 alleles per locus, 0.25-0.80 observed heterozygosity (Ho), and 0.30-0.80 expected heterozygosity (He). All loci were in Hardy-Weinberg equilibrium and linkage equilibrium and many loci amplified and were polymorphic in related taxa.
CONCLUSIONS
These loci will be used to identify endemic populations of common raven and assess their genetic diversity and connectivity.
背景
保护工作的一个重点是识别特有种群。我们开发了用于普通渡鸦（Corvus corax）的微卫星标记，普通渡鸦是一种分布于全北区的鸟类，旨在识别和评估阿拉斯加的特有种群。
结果
从总共50个微卫星位点中，我们分离并鉴定了15个位点。其中8个位点具有多态性且易于评分。对来自阿拉斯加费尔班克斯的18至20只普通渡鸦进行基因分型，结果显示出以下变异性：每个位点有3至8个等位基因，观察到的杂合度（Ho）为0.25至0.80，预期杂合度（He）为0.30至0.80。所有位点均处于哈迪-温伯格平衡和连锁平衡状态，并且许多位点在相关分类群中能够扩增且具有多态性。
结论
这些位点将用于识别普通渡鸦的特有种群，并评估其遗传多样性和连通性。
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