Department of Chemistry, Fudan University, 220 Han Dan Road, Shanghai, 200433, China; Institutes of Biomedical Sciences, Fudan University, 138 Yi Xue Yuan Road, Shanghai, 200032, China.
Cytometry A. 2011 Oct;79(10):848-54. doi: 10.1002/cyto.a.21134. Epub 2011 Sep 13.
In metastasis, the cancer cells that travel through the body are capable of establishing new tumors in locations remote from the site of the original disease. To metastasize, a cancer cell must break away from its tumor and invade either the circulatory or lymphatic system, which will carry it to a new location, and establish itself in the new site. Once in the blood stream, the cancer cells now have access to every portion of the body. Here, we have used the "in vivo flow cytometer" to study if there is any relationship between metastatic potential and depletion kinetics of circulating tumor cells. The in vivo flow cytometer has the capability to detect and quantify continuously the number and flow characteristics of fluorescently labelled cells in vivo. We have improved the counting algorithm and measured the depletion kinetics of cancer cells with different metastatic potential. Interestingly, more invasive PC-3 prostate cancer cells are depleted faster from the circulation than LNCaP cells. In addition, we have measured the depletion kinetics of two related human hepatocellular carcinoma (liver cancer) cell lines, high-metastatic HCCLM3 cells, and low-metastatic HepG2 cells. More than 60% HCCLM3 cells are depleted within the first hour. Interestingly, the low-metastatic HepG2 cells possess noticeably slower depletion kinetics. In comparison, <40% HepG2 cells are depleted within the first hour. The differences in depletion kinetics might provide insights into early metastasis processes.
在转移过程中，能够通过身体传播的癌细胞能够在远离原始疾病部位的地方建立新的肿瘤。为了转移，癌细胞必须从肿瘤中分离出来并侵入循环系统或淋巴系统，这将把它带到新的位置，并在新的位置建立自己。一旦进入血液，癌细胞现在就可以进入身体的各个部位。在这里，我们使用“活体流式细胞仪”来研究转移潜能和循环肿瘤细胞耗竭动力学之间是否存在任何关系。活体流式细胞仪具有连续检测和定量标记荧光细胞数量和流动力学特性的能力。我们改进了计数算法，并测量了具有不同转移潜能的癌细胞的耗竭动力学。有趣的是，侵袭性更强的 PC-3 前列腺癌细胞比 LNCaP 细胞从循环中更快地被消耗。此外，我们还测量了两种相关的人肝癌（肝癌）细胞系、高转移性 HCCLM3 细胞和低转移性 HepG2 细胞的耗竭动力学。超过 60%的 HCCLM3 细胞在第一个小时内被消耗。有趣的是，低转移性 HepG2 细胞的消耗动力学明显较慢。相比之下，<40%的 HepG2 细胞在第一个小时内被消耗。耗竭动力学的差异可能为早期转移过程提供了一些见解。
