Z Naturforsch C J Biosci. 2011 Jul-Aug;66(7-8):328-32. doi: 10.1515/znc-2011-7-802.
Nannochloropsis salina was cultured batch-wise to evaluate the potential of the alga to produce biodiesel. The cells were harvested at the end of the exponential growth phase when the concentration was 18 x 10(6) cells/mL culture. The growth estimated as dry weight from this cell number was (3.8 +/- 0.7) mg/L. The lipid and triglyceride contents were 40% and 12% on a dry weight basis, respectively. The amount of the ratio triglycerides/total lipids was approximately 0.3. The composition of triglyceride fatty acid methyl esters (biodiesel) was analysed by gas-liquid chromatography and identified as: C14:0, C16:0, C16:1, C18:0, C18:1, C18:2, C18:3, C20:1, and C20:5. The ratio of unsaturated to saturated fatty acid contents was approximately 4.4. Additionally, the characterization of each individual fatty acid ester was discussed with regard to the fuel properties of biodiesel produced by the alga.
小球藻分批培养，以评估藻类生产生物柴油的潜力。当细胞浓度达到 18×10(6)个细胞/ml 培养时，在指数生长期结束时收获细胞。从这个细胞数估计的生长量以干重计为 (3.8±0.7)mg/L。脂质和三酰基甘油的含量分别为干重的 40%和 12%。三酰基甘油/总脂质的比例约为 0.3。通过气相色谱- 液相色谱分析鉴定了三酰基甘油脂肪酸甲酯（生物柴油）的组成：C14:0、C16:0、C16:1、C18:0、C18:1、C18:2、C18:3、C20:1 和 C20:5。不饱和脂肪酸与饱和脂肪酸的比例约为 4.4。此外，还讨论了每个脂肪酸酯的特性，以及藻类生产的生物柴油的燃料特性。
