人唾液（糖）蛋白单克隆抗体的特性。人下颌下腺中黏蛋白、胱抑素样14kD蛋白和20kD糖蛋白的细胞定位。

Characterization of monoclonal antibodies to human salivary (glyco) proteins. Cellular localization of mucin, cystatin-like 14 kD protein and 20 kD glycoprotein in the human submandibular gland.

作者信息

Rathman W M, van den Keybus P A, van Zeyl M J, Döpp E A, Veerman E C, Nieuw Amerongen A V

机构信息

Dept. of Oral Biochemistry, Academic Centre for Dentistry (ACTA), Amsterdam.

出版信息

J Biol Buccale. 1990 Mar;18(1):19-27.

Abstract

Using the hybridoma technique, monoclonal antibodies (Mabs) have been produced against three different types of human salivary proteins: high molecular weight mucin, a 20 kD glycoprotein and a 14 kD protein, identified as a member of the cystatin family. The Mabs appeared to be highly specific to their antigen in Elisa and immunoblotting tests. The Mabs were of the IgG-1 (against 20 kD glycoprotein) and IgM (against 14 kD protein and mucin) type. For the 14 kD protein and the 20 kD glycoprotein it was demonstrated that they are present mainly in submandibular-sublingual saliva. None of the antigens studied could be localized distinctly in the human parotid gland. In the submandibular gland, the three proteins have a different pattern of localization. The mucins have been detected particularly in the apical part of the mucous acinar cells, the 20 kD glycoprotein mainly in the serous acinar cells and the 14 kD protein in both serous acinar cells and striated duct cells.

摘要

利用杂交瘤技术，已制备出针对三种不同类型人唾液蛋白的单克隆抗体（Mabs）：高分子量粘蛋白、一种20kD糖蛋白和一种14kD蛋白，后者被鉴定为胱抑素家族的一员。在酶联免疫吸附测定（ELISA）和免疫印迹试验中，这些单克隆抗体似乎对其抗原具有高度特异性。这些单克隆抗体属于IgG - 1型（针对20kD糖蛋白）和IgM型（针对14kD蛋白和粘蛋白）。对于14kD蛋白和20kD糖蛋白，已证实它们主要存在于颌下腺 - 舌下腺唾液中。所研究的抗原在人腮腺中均无法明显定位。在颌下腺中，这三种蛋白质具有不同的定位模式。粘蛋白尤其在粘液腺泡细胞的顶端部分被检测到，20kD糖蛋白主要在浆液腺泡细胞中，而14kD蛋白在浆液腺泡细胞和纹状管细胞中均有发现。