使用单克隆抗体对高分子量人唾液粘蛋白（MG1）进行免疫化学分析。

Immunochemical analysis of high molecular-weight human salivary mucins (MG1) using monoclonal antibodies.

作者信息

Veerman E C, Valentijn-Benz M, van den Keybus P A, Rathman W M, Sheehan J K, Nieuw Amerongen A V

机构信息

Department of Oral Biochemistry, Academic Centre for Dentistry Amsterdam (ACTA), Vrije Universiteit, The Netherlands.

出版信息

Arch Oral Biol. 1991;36(12):923-32. doi: 10.1016/0003-9969(91)90125-e.

DOI:10.1016/0003-9969(91)90125-e

PMID:1722666

Abstract

Using four Mabs with different specificities for salivary mucins, an ELISA has been developed in which human whole saliva, glandular salivas, salivary protein fractions and purified, high molecular-weight, mucin fractions (MG1) isolated from human submandibular and sublingual glandular tissues have been immunochemically analysed. All four Mabs reacted with MG1s. Three of them reacted with the purified, low molecular-weight salivary mucins (MG2). None was reactive with parotid saliva. MG1 preparations isolated from submandibular and sublingual glandular tissues of one and the same individual displayed different patterns of reactivity with these Mabs, indicating that they differ immunochemically. Analysis of the MG1s in salivas derived from individual salivary glands showed differences in immunochemical composition. These results indicate that the MG1 fraction in human whole saliva consists of several immunochemically different species.

摘要

利用针对唾液粘蛋白具有不同特异性的四种单克隆抗体，开发了一种酶联免疫吸附测定法（ELISA），用于对人全唾液、腺性唾液、唾液蛋白组分以及从人颌下腺和舌下腺组织中分离出的纯化高分子量粘蛋白组分（MG1）进行免疫化学分析。所有四种单克隆抗体均与MG1发生反应。其中三种与纯化的低分子量唾液粘蛋白（MG2）发生反应。无一与腮腺唾液发生反应。从同一个体的颌下腺和舌下腺组织中分离出的MG1制剂与这些单克隆抗体表现出不同的反应模式，表明它们在免疫化学上存在差异。对来自个体唾液腺的唾液中MG1的分析显示出免疫化学组成上的差异。这些结果表明，人全唾液中的MG1组分由几种免疫化学上不同的种类组成。