Smits K, Govaere J, Hoogewijs M, Piepers S, Van Soom A
Department of Reproduction, Obstetrics and Herd Health, Ghent University, Merelbeke, Belgium.
Reprod Domest Anim. 2012 Feb;47(1):e1-3. doi: 10.1111/j.1439-0531.2011.01814.x. Epub 2011 Sep 24.
Intracytoplasmic sperm injection (ICSI) is the method of choice for the in vitro production (IVP) of equine embryos. However, conventional ICSI has been associated with mechanical damage to the oocyte caused by the deformation of the zona pellucida (ZP) and exposure of the oolemma to negative pressure during injection. Introduction of the less traumatic and more efficient piezo drill-assisted ICSI (PDAI) yielded higher cleavage rates and more consistent results. Nevertheless, PDAI is also associated with disadvantages such as the use of mercury and possible DNA damage. This led us to explore an alternative method avoiding oocyte trauma, namely laser-assisted ICSI (LAI), which involves creating a hole in the ZP prior to ICSI. In this pilot study, PDAI and LAI were compared for ICSI in the horse. No significant influences on subsequent embryonic development were observed.
胞浆内单精子注射（ICSI）是马胚胎体外生产（IVP）的首选方法。然而，传统的ICSI与卵母细胞的机械损伤有关，这种损伤是由透明带（ZP）变形以及注射过程中卵膜暴露于负压引起的。创伤性较小且效率更高的压电钻辅助ICSI（PDAI）的引入产生了更高的卵裂率和更一致的结果。然而，PDAI也存在一些缺点，如使用汞以及可能的DNA损伤。这促使我们探索一种避免卵母细胞创伤的替代方法，即激光辅助ICSI（LAI），它涉及在ICSI之前在ZP上创建一个孔。在这项初步研究中，对马的ICSI中PDAI和LAI进行了比较。未观察到对后续胚胎发育有显著影响。
