Zhang Yong, Han Duo-Hong, Jin Ling, Wang Sheng-Qing
College of Agriculture and Biotechnology, Hexi University, Zhangye 734000, China.
Zhongguo Zhong Yao Za Zhi. 2012 Oct;37(20):3036-40.
In order to get the method for improving the salt resistance of Hedysarum polybotrys seeds and seedlings under different salt-alkaline stress, the seed germination and physiological characteristics of H. polybotrys seedlings were studied.
Several physiological indexes of H. polybotrys seeds under different salt-alkaline stress, such as the germination vigor, germination rate, relative germination rate, relative salt damage rate were measured. And others indexes of the seedlings like chlorophyll contents, soluble protein contents, the permeability of plasmalemma, the activities of POD and SOD were also measured.
Different salt-alkaline stress decreased the germination rate, vigor of germinate, germination index, while relative salt damage rate increased. With the increased salt-alkaline concentration, the adverse effects became more obvious. The strength of the salts: Na2CO3 > Na2SO4 > NaCl. With the increase of the salt-alkaline concentration, the chlorophyll contents and the soluble protein contents decreased, but the permeability of plasmalemma increased. The change trend of SOD and POD activity was similar, it is increased firstly, and then decreased as the stress intensity extended, the most significant increase of Na2SO4 and Na2CO3 in the concentration of salt-alkaline was 25 mmol x L(-1), but NaCl was 50 mmol x L(-1).
The seeds and seedlings inhibition of the salts was Na2CO3 > Na2SO4 > NaCl.
为探寻提高多花黄芪种子和幼苗在不同盐碱胁迫下耐盐性的方法，对多花黄芪种子萌发及幼苗生理特性进行研究。
测定多花黄芪种子在不同盐碱胁迫下的多项生理指标，如发芽势、发芽率、相对发芽率、相对盐害率等。同时测定幼苗的其他指标，如叶绿素含量、可溶性蛋白含量、质膜透性、过氧化物酶（POD）和超氧化物歧化酶（SOD）活性等。
不同盐碱胁迫降低了多花黄芪种子的发芽率、发芽势和发芽指数，相对盐害率升高。随着盐碱浓度增加，不利影响更明显。盐害强度顺序为：Na₂CO₃＞Na₂SO₄＞NaCl。随着盐碱浓度增加，叶绿素含量和可溶性蛋白含量降低，质膜透性增加。SOD和POD活性变化趋势相似，先升高，随着胁迫强度增大后降低，Na₂SO₄和Na₂CO₃在盐碱浓度为25 mmol·L⁻¹时活性增加最显著，而NaCl在50 mmol·L⁻¹时活性增加最显著。
多花黄芪种子和幼苗对盐害的耐受能力为Na₂CO₃＞Na₂SO₄＞NaCl。
