Kiros Tadele G, van Kessel Jill, Babiuk Lorne A, Gerdts Volker
Vaccine and Infectious Disease Organization, University of Saskatchewan, 120 Veterinary Road, Saskatoon, SK S7N 5E3, Canada.
Vet Immunol Immunopathol. 2011 Dec 15;144(3-4):448-54. doi: 10.1016/j.vetimm.2011.08.021. Epub 2011 Sep 10.
Interleukin-17 (IL-17) producing cells, referred to as Th17, have recently emerged as a third subset of the T helper (Th) cell family. Studies in mice have demonstrated that Th17 cells and their associated cytokines are involved in several autoimmune diseases and host defense against infection. Murine Th17 cells differentiate from naïve CD4(+) T-cells in the presence of TGFβ and IL-6, however, there are contradicting reports as to the role of TGFβ in the differentiation of human Th17 cells and very little is known about these cells in other animals. We report here the presence of IL-17 secreting lymphocytes in the lung and peripheral blood of pigs. The cDNA of porcine IL-17 gene was cloned and sequenced from activated lung lymphocytes and PBMC from piglets. A 17kDa recombinant protein was expressed and purified both under denaturing and native conditions from E. coli BL21 cells. Furthermore, we demonstrate that TGFβ in the presence of IL-6 and/or IL-1β induces in vitro differentiation of Th17 cells from naïve porcine CD4(+) thymocytes.
产生白细胞介素-17（IL-17）的细胞，即Th17细胞，最近已成为辅助性T（Th）细胞家族的第三个亚群。小鼠研究表明，Th17细胞及其相关细胞因子参与多种自身免疫性疾病以及宿主抗感染防御。在转化生长因子β（TGFβ）和白细胞介素-6（IL-6）存在的情况下，小鼠Th17细胞从初始CD4(+) T细胞分化而来，然而，关于TGFβ在人类Th17细胞分化中的作用存在相互矛盾的报道，并且对于其他动物中的这些细胞知之甚少。我们在此报告猪的肺和外周血中存在分泌IL-17的淋巴细胞。从仔猪的活化肺淋巴细胞和外周血单核细胞（PBMC）中克隆并测序了猪IL-17基因的cDNA。在变性和天然条件下，从大肠杆菌BL21细胞中表达并纯化了一种17kDa的重组蛋白。此外，我们证明在IL-6和/或IL-1β存在的情况下，TGFβ可诱导初始猪CD4(+)胸腺细胞在体外分化为Th17细胞。
