伊洛前列素对大鼠结肠吻合口愈合的影响。

The effects of iloprost on colonic anastomotic healing in rats.

机构信息

4th Surgical Department, Aristotle University of Thessaloniki, 2 Macedonias Str, PO 55535, Pilea, Thessaloniki, Greece.

出版信息

Tech Coloproctol. 2011 Oct;15 Suppl 1:S117-20. doi: 10.1007/s10151-011-0758-5.

DOI:10.1007/s10151-011-0758-5

PMID:21956403

Abstract

PURPOSE

The purpose of this experimental study is to investigate the effects of iloprost on colonic anastomotic healing in rats, after intraperitoneal administration.

METHODS

Forty male Albino-Wistar rats were randomized into two groups of twenty animals each. They all underwent colonic resection followed by an inverted anastomosis. The rats of Group A (control) received 3 ml of NaCl intraperitoneally, while those of Group B (iloprost) received iloprost (2 μg/kg body weight), immediately postoperatively and daily until killed. Each group was further divided into two equal subgroups, depending on the day of killing. The animals of subgroups 1 were killed on the fourth postoperative day, while those of subgroups 2 on the eighth. Macroscopical and histological assessments were performed. Besides, anastomotic bursting pressures and the tissue concentrations in hydroxyproline and collagenase I were also evaluated.

RESULTS

No anastomotic dehiscence was noted. The mean bursting pressure was higher in the iloprost group compared with the control group, but a significant difference was revealed only on the fourth postoperative day. Furthermore, iloprost significantly increased the new vessel formation on the fourth, as well as on the eighth postoperative day.

CONCLUSION

Iloprost enhances the early phase of colonic anastomotic healing in rats.

摘要

目的

本实验研究的目的是探讨伊洛前列素经腹腔给药对大鼠结肠吻合口愈合的影响。

方法

40 只雄性白化 Wistar 大鼠随机分为两组，每组 20 只。所有大鼠均接受结肠切除术和反向吻合术。A 组（对照组）大鼠腹腔内给予 3ml NaCl，B 组（前列环素组）大鼠术后立即给予前列环素（2μg/kg 体重），每天一次，直至处死。每组再根据处死日分为两组。第 1 亚组的动物在术后第 4 天被处死，第 2 亚组的动物在术后第 8 天被处死。进行大体和组织学评估。此外，还评估了吻合口爆裂压和羟脯氨酸及胶原酶 I 的组织浓度。