Ahlborg Niklas, Axelsson Bernt
MABTECH AB, Nacka Strand, Sweden.
Methods Mol Biol. 2012;792:77-85. doi: 10.1007/978-1-61779-325-7_6.
Cytokine ELISPOT has become a powerful routine tool for the analysis of disease- as well as vaccine-induced T-cell responses. The method is limited, however, in that only one cytokine at a time is assessed. Fluorospot is based on the principle of ELISPOT, but facilitates the analysis of single cells secreting several cytokines, e.g., polyfunctional T cells, suggested to be of protective importance in various infectious diseases. By detecting each cytokine with a specific fluorophore and analyzing differentially colored spots by fluorophore--specific filter systems, cells producing single or multiple cytokines are identified. Fluorospot maintains the simplicity and sensitivity of the ELISPOT while taking the analysis a step forward toward multiplex analysis.
细胞因子酶联免疫斑点技术（Cytokine ELISPOT）已成为分析疾病以及疫苗诱导的T细胞反应的强大常规工具。然而，该方法存在局限性，即一次只能评估一种细胞因子。荧光免疫斑点技术（Fluorospot）基于酶联免疫斑点技术的原理，但有助于分析分泌多种细胞因子的单个细胞，例如多功能T细胞，这些细胞在各种传染病中被认为具有重要的保护作用。通过用特定荧光团检测每种细胞因子，并通过荧光团特异性滤光系统分析不同颜色的斑点，可识别产生单一或多种细胞因子的细胞。荧光免疫斑点技术在保持酶联免疫斑点技术简单性和敏感性的同时，使分析向多重分析迈进了一步。
