He Jie, Qiu Weiliu, Zhang Zhiyuan, Wang Zhonghe, Zhang Xiuli, He Yue
Department of Oral and Maxillofacial Surgery, Shanghai Jiao-tong University, School of Medicine, Shanghai Ninth People's Hospital, Shanghai, People's Republic of China.
J Craniofac Surg. 2011 Sep;22(5):1635-40. doi: 10.1097/SCS.0b013e31822e5f66.
Osteoblasts are bone-forming cells that are responsible for the production of bone extracellular matrix. Osteoradionecrosis is a complication of radiation therapy for carcinoma of the head and neck that occurs in 3% to 8.2% of irradiated patients. The irradiation effect on osteoblast differentiation has not been fully elucidated. The objective of our research was to elucidate the effects of radiation on the growth and differentiation-related gene expression in osteoblast in vitro. Three differentiation-related genes, alkaline phosphatase, type I collagen, and osteocalcin, were tested in our experiment. The results showed that radiation inhibited the proliferation of the osteoblasts in a dose-dependent manner and induced G2/M cell cycle arrest. Irradiation, 4 and 8 Gy, enhanced the differentiation-related gene expression of MC3T3-E1 cells 7 days after irradiation. However, the differentiation-related gene expression was decreased 21 days after irradiation in the 4- and 8-Gy groups. This work presents the dynamic phenotypic expression changes of osteoblastic cells after x-ray irradiation.
成骨细胞是负责产生骨细胞外基质的骨形成细胞。放射性骨坏死是头颈部癌放射治疗的一种并发症，在接受放射治疗的患者中发生率为3%至8.2%。辐射对成骨细胞分化的影响尚未完全阐明。我们研究的目的是阐明辐射对体外成骨细胞生长和分化相关基因表达的影响。在我们的实验中检测了三个与分化相关的基因，即碱性磷酸酶、I型胶原和骨钙素。结果表明，辐射以剂量依赖的方式抑制成骨细胞的增殖并诱导G2/M期细胞周期阻滞。4 Gy和8 Gy照射在照射后7天增强了MC3T3-E1细胞的分化相关基因表达。然而，在4 Gy和8 Gy组中，照射后21天分化相关基因表达下降。这项工作展示了X射线照射后成骨细胞的动态表型表达变化。
